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Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907)

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Overview

  • Product nameThrombin-Antithrombin Complex (TAT) Human ELISA KitSee all Thrombin-Antithrombin Complex kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.48 ng/ml - 120 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Thrombin-antithrombin (TAT) complexes formed following the neutralization of thrombin by antithrombin III (AT) have been used as a surrogate marker for thrombin generation. High plasma level of TAT complexes has been suggested to alter hemostatic activation in argentine hemorrhagic fever, chronic dialysis patients, and toxemia of pregnancy. Whereas low plasma level of TAT complexes is found in type 1 (insulin-dependent) diabetes, neonatal respiratory distress syndrome, and primary untreated cancer. TAT complexes are a useful marker to predict morphological changes in chronic aortic dissection.

    ab108907 Thrombin-antithrombin Complex Human ELISA kit is designed for detection of human TAT complexes in plasma, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TAT complexes in less than 4 hours. A monoclonal antibody specific for Antithrombin has been pre-coated onto a microplate. TAT complexes in standards and samples are sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Thrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • RelevanceThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localizationSecreted
    • Alternative names
        antithrombin IIIAT3AT3D
        ATIIIcoagulation factor II (thrombin)F2PRO0309prothrombinprothrombin B chainPTRPRGL2serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1serine proteaseserine-cysteine proteinase inhibitor clade C member 1serpin C1serpin peptidase inhibitor, clade C (antithrombin), member 1SERPINC1TATTHPH1THPH7
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108907 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Thrombin-Antithrombin Complex (TAT) Human ELISA Kit images

      • ELISA - Thrombin-antithrombin Complex Human ELISA Kit (ab108907)
        ELISA - Thrombin-antithrombin Complex Human ELISA Kit (ab108907)
        Human Thrombin-antithrombin (TAT) Complexes Standard Curve

      References for Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907)

      ab108907 has not yet been referenced specifically in any publications.

      Publishing research using ab108907 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"