Thrombin Human ELISA Kit (ab108909)
- Product nameThrombin Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant
- Assay typeSandwich
- Sensitivity= 0.3 ng/ml
- Range0.313 ng/ml - 20 ng/ml
- Assay time4h 0m
- Species reactivityReacts with: Human
- Product overview
Thrombin (activated Factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease (EC 22.214.171.124) that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin, that is the immediate end product of prothrombin activation, two further thrombin products can be identified: beta- and gamma-thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation.
ab108909 Thrombin Human ELISA kit is designed for detection of alpha Thrombin in Human cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Thrombin in 4 hours. A monoclonal antibody specific for Thrombin has been pre-coated onto a microplate. Thrombin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Thrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
ab108909 recognizes both natural and recombinant Human Thrombin, and has about 70% cross reactivity to Human prothrombin.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Thrombin Antibody (80x) 1 x 100µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Thrombin Microplate 1 unit Thrombin Standard 1 unit Wash Buffer Concentrate (20x) 1 x 30ml
- SwissProt: P00734 Human
Our Abpromise guarantee covers the use of ab108909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Thrombin Human ELISA Kit (ab108909)
This product has been referenced in:
- Liu JF et al. Thrombin induces heme oxygenase-1 expression in human synovial fibroblasts through protease-activated receptor signaling pathways. Arthritis Res Ther 14:R91 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22541814) »