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Overview

  • Product nameThrombin Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich
  • Sensitivity
    = 0.3 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    95 %

  • Assay time
    4h 0m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Thrombin (activated Factor II [IIa]) is a coagulation protein that has many effects in the coagulation cascade. Thrombin is a serine protease (EC 3.4.21.5) that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. Thrombin is in the form of alpha-thrombin, that is the immediate end product of prothrombin activation, two further thrombin products can be identified: beta- and gamma-thrombin. These are degraded forms that may arise from autodigestion of a thrombin preparation.

    ab108909 Thrombin Human ELISA kit is designed for detection of alpha Thrombin in Human cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Thrombin in 4 hours. A monoclonal antibody specific for Thrombin has been pre-coated onto a microplate. Thrombin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Thrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

     

    ab108909 recognizes both natural and recombinant Human Thrombin, and has about 70% cross reactivity to Human prothrombin.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108909 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Thrombin Human ELISA Kit images

      • Sandwich ELISA - Thombin Human ELISA Kit (ab108909)
        Sandwich ELISA - Thombin Human ELISA Kit (ab108909)
        Human Thombin Standard Curve

      Protocols

      References for Thrombin Human ELISA Kit (ab108909)

      This product has been referenced in:
      • Liu JF  et al. Thrombin induces heme oxygenase-1 expression in human synovial fibroblasts through protease-activated receptor signaling pathways. Arthritis Res Ther 14:R91 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22541814) »

      See 1 Publication for this product

      Publishing research using ab108909 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit a Question for ab108909

      Displaying 1 - 4 of 4 results for Q&A

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      Votes: Highest First

      I received the abcam Thrombin Elisa Assay today. In anticipation of my experiment next week, I wanted to make the wash buffer solution. Instead of adding the 20X wash buffer concentrate to the dH2O, I added 10 mLs of the 10X diluent instead. I wanted to k...

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      Thank you for your patience while I looked into this.

      The lab has sent us thereplacement vials of the diluent and wash buffer. We are sending these to you free of charge on order. You should receive these tomorrow.

      I hope this informat...

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      How shall I reconstitute the standard? The lot number is GR88036-1.

      Thank you for contacting Abcam regarding ab108909.


      Just to confirm what we discussed over the phone:


      Thrombin Standard: Purified human Thrombin in a buffered protein base (40 ng, lyophilized).

      Standard Curve: Reconstit...

      Read More

      I received the abcam Thrombin Elisa Assay today. In anticipation of my experiment next week, I wanted to make the wash buffer solution. Instead of adding the 20X wash buffer concentrate to the dH2O, I added 10 mLs of the 10X diluent instead. I wanted to k...

      Read More

      Thank you for contacting us.

      I heard back from the lab and they recommend that youdispose the Wash Buffer and the Diluent if you mixed them together.

      Wash Buffer is 20x and should be diluted with water (1 part Wash Buffer + 19 parts of...

      Read More

      Is there any proteins in the storage buffer on the Thrombin standard that would run at about the same size as the thrombin protein itself?

      Thank you for calling Abcam earlier today.
      I have talked to the lab and they do not think there is protein the same size as thrombin in the buffer based, but we cannot be 100% sure. The exact components of the buffer are proprietary and so I am unable...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"