Recombinant Anti-Hsp47 antibody [EPR4217] (ab109117)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4217] to Hsp47
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp47 antibody [EPR4217]
See all Hsp47 primary antibodies -
Description
Rabbit monoclonal [EPR4217] to Hsp47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
For unpurified use at 1/100. |
IHC-P | (1) |
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. For unpurified use at 1/100. |
IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Target
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Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
Involvement in disease
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 871 Human
- Entrez Gene: 12406 Mouse
- Entrez Gene: 29345 Rat
- Omim: 600943 Human
- SwissProt: P50454 Human
- SwissProt: P19324 Mouse
- SwissProt: P29457 Rat
- Unigene: 596449 Human
see all -
Alternative names
- 47 kDa heat shock protein antibody
- 47 kDa heat shock protein precursor antibody
- Arsenic transactivated protein 3 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109117 observed at 46 kDa. Red - loading control, ab9484, observed at 37 kDa.ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab109117 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
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All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution
Lane 1 : A431 Cell Lysate
Lane 2 : NIH/3T3 Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 46 kDa -
All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab109117 has been referenced in 36 publications.
- Murphy C et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
- Bons J et al. Data-independent acquisition and quantification of extracellular matrix from human lung in chronic inflammation-associated carcinomas. Proteomics 23:e2200021 (2023). PubMed: 36228107
- Ackerman JE et al. Defining the spatial-molecular map of fibrotic tendon healing and the drivers of Scleraxis-lineage cell fate and function. Cell Rep 41:111706 (2022). PubMed: 36417854
- Herrera JA et al. The UIP/IPF fibroblastic focus is a collagen biosynthesis factory embedded in a distinct extracellular matrix. JCI Insight 7:N/A (2022). PubMed: 35852874
- Li M et al. Regulation of the unfolded protein response transducer IRE1α by SERPINH1 aggravates periodontitis with diabetes mellitus via prolonged ER stress. Cell Signal 91:110241 (2022). PubMed: 34998932