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Overview

  • Product nameDRAQ7™ 1ml (0.3mM)
  • Tested applicationsFluorescence Microscopy, Flow Cyt, ICC/IF more details
  • General notesDRAQ7™ is a new far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC. DRAQ7™ is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. DRAQ7™ is an ideal replacement for propidium iodide (and 7-AAD), having far better spectral properties; it has no UV excitation and no emission overlap with PE and homologues.

    Key features of DRAQ7™ include:
    • Rapid staining of dsDNA/ nuclei of DEAD or permeabilized cells
    • Low photobleaching effect
    • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
    • Non-toxic in long-term culture
    • Can be combined with "live" dyes
    • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
    • No wash or RNase treatment needed.

    SPECTRAL PROPERTIES Excitation:

    • 633 and 647 nm line optimal (Exmax 599 / 644 nm)
    • 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)

    Emission (instrument dependent):

    • 665 nm to infra-red <800 nm (Emmax 678 nm / 697 nm intercalated with dsDNA)
    • Minimal overlap with vis range e.g. GFP and FITC
    • Em. filters may include 695L, 715LP or 780 LP

    Multi-wavelength imaging with UV / vis fluorochromes

    • No fluorescence enhancement upon DNA binding
    • Low photo-bleaching effect
    • Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab109202 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy: Use at an assay dependent concentration.
    Flow Cyt Flow Cyt: 1/100. (final concentration = 3µM)
    ICC/IF ICC/IF: 1/100. (final concentration = 3µM)
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.

    DRAQ7™ 1ml (0.3mM) images

    • Flow Cytometry - DRAQ7™ 1ml (0.3mM) (ab109202)
      Flow Cytometry - DRAQ7™ 1ml (0.3mM) (ab109202)
      Jurkat cells exposed to 1µM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.
    • Immunohistochemistry (Frozen sections) - DRAQ7™ 1ml (0.3mM) (ab109202)
      Immunohistochemistry (Frozen sections) - DRAQ7™ 1ml (0.3mM) (ab109202)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

      Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
      Preparation:

      • Fix in 3%PFA in PBS for 30 min at RT
      • Incubate in 7.5% sucrose-PBS for 3h at RT
      • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
      • Embed the EBs in tissue-Tek OCT compound
      • Cut frozen sections to 4-20 µm thickness

      Primary antibody: Rabbit anti-laminin alpha 1, 1:400
      Secondary antibody: Goat anti-rabbit IgG - H&L (AMCA) (ab123435)
      Nuclei were counterstained stained with DRAQ7™ (ab109202)

    • Immunohistochemistry (Frozen sections) - DRAQ7™ 1ml (0.3mM) (ab109202)
      Immunohistochemistry (Frozen sections) - DRAQ7™ 1ml (0.3mM) (ab109202)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

      Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

      Preparation:

      • Fix in 3%PFA in PBS for 30 min at RT
      • Incubate in 7.5% sucrose-PBS for 3h at RT
      • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
      • Embed the EBs in tissue-Tek OCT compound
      • Cut frozen sections to 4-20 µm thickness


      Primary antibody: Rabbit anti-laminin alpha 1, 1:400
      Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:200
      F-actin was stained with       CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
      Nuclei were counterstained stained with DRAQ7TM, 1:1000

    Protocols

    References for DRAQ7™ 1ml (0.3mM) (ab109202)

    ab109202 has not yet been referenced specifically in any publications.

    Publishing research using ab109202 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab109202 Submit a Question for ab109202

    Displaying 1 - 8 of 8 results for Abreviews and Q&A

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    Votes: Highest First

    Toxicity in comparison to DAPI

    The bottom line is that toxicity studies have yet to been done on DAPI. I have seen one article questioning its use (Qin, 2011 using it on MSCs).
    DAPI is known to be semi-permeant to cells and thus it does get in. As a very high affinity DNA binding...

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    Hi Abcam,

    Would you please be able to send a copy of the MSDS for this product to me,

    The link on the webpage datasheet is not working.

    Thanks for your help and hope to hear from you soon

    Kind regards,

    Thank you for your enquiry.

    My colleagues have just uploaded the SDS document so your customer could get access to the requested form by clinking on the hyperlink.

    If you need any further assistance in the future, please do not hesitat...

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    Hi Abcam,

    Can you please help with an enquiry we have received from a customer;
    DRAQ7™ 1ml (0.3mM) (ab109202)

    I would like to get some recommendation for a compatible fluorescent dye to stain all (live and dead) cells to be us...

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    Thank you for contacting us.

    We have several products from the CytoPainter range which may be of interest to your customer. These can be accessed from the following link:

    CytoPainter staining kits | Abcam

    These kits allow for th...

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    Is Draq7TM capable of distiguishing diploid from tertraploid and octoploid nuclei by FACS? We are currently using propidium iodide, which works well but overlaps with the emission of our Tomato Red co-stain. We do not permeabilize the nuclei but have foun...

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    Thank you for your enquiry.

    We recommend Draq5TM, ab108410, for your application, rather than Draq7TM. The laboratory has provided the following notes which describe the differences between the two. In brief, the max emission wavelengths are th...

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    Dear Tech Support Team, Kindly advise if DRAQ5 and DRAQ7 are suitable for yeast. Thanks in advance foryour assistance and reply. Kind Regards,

    Thank you for your patience. I have now received the answer in regards to the use of DRAQ7 on yeast. Unfortunately the laboratory does not know neither whether it will work on yeast cells, due to the difference in the cell wall. I am very sorry therefor...

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    Kindly advise if DRAQ5 and DRAQ7 are suitable for yeast. Thanks in advance foryour assistance and reply.

    Thank you for contacting us. Indeed, DRAQ5 has already been used successfully in yeast already. Please see here below the publication citing it: DRAQ5 - http://aem.asm.org/content/72/10/6725.abstract I expect DRAQ7 to be suitable as well for yeast, as it...

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    I'm using DRAQ7 and would like to stain for live/dead fixed cells in microscopy. According to the protocol, DRAQ7 will stain all fixed and permeabilized cells in the preparation. How can I then differentiate between my live and dead cells if all are goi...

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    Thank you for your inquiry. DRAQ7 is a LIVE cell impermeant far-red DNA dye. It can be used to identify permeable cells in a mix of live and dead cells (i.e. the dead cells) – but in a preparation of fixed cells, all of the cells will be permeant and t...

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    24 h experiment to detect live cells, no additional treatment which dye best to use? Draq5 (for live cells) - too toxic Draq7 is for staining dead and permeabilized cells

    Thank you for contacting us. I have received the following response from the lab: We would suggest to approach this as follows ... Treat the cell culture with 1-3 uM DRAQ7 during the incubation, image to enumerate the dead/leaky cells at the req...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"