Recombinant Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2580(2)] to PIAS1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PIAS1 antibody [EPR2580(2)]
See all PIAS1 primary antibodies -
Description
Rabbit monoclonal [EPR2580(2)] to PIAS1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates. ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2580(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/500.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
|
Notes |
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ICC/IF
1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. -
Tissue specificity
Expressed in numerous tissues with highest level in testis. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
Domain
The LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation. -
Post-translational
modificationsSumoylated.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene. -
Cellular localization
Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 8554 Human
- Entrez Gene: 56469 Mouse
- Entrez Gene: 300772 Rat
- Omim: 603566 Human
- SwissProt: O75925 Human
- SwissProt: O88907 Mouse
- Unigene: 162458 Human
- Unigene: 431253 Mouse
see all -
Alternative names
- AR interacting protein antibody
- DDXBP1 antibody
- DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
see all
Images
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Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution
All lanes : Recombinant Human PIAS1 protein (ab152888)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 10 seconds.
Lane 2: 1 minute.
Blocking and dilution buffer: 5% NFDM /TBST.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PIAS1 with purified ab109388 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
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All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : HEK-293 cell lysates
Lane 4 : Daudi cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab109388 has been referenced in 16 publications.
- Lee YH et al. A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Mov Disord 37:767-777 (2022). PubMed: 34951052
- Wang G et al. PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence. PLoS Pathog 18:e1010446 (2022). PubMed: 35377920
- Huang M et al. NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination. Aging (Albany NY) 14:3529-3539 (2022). PubMed: 35460552
- Chen Q et al. TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease. Front Physiol 12:709506 (2021). PubMed: 34434118
- Chen Z et al. Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z. J Biol Chem 297:101200 (2021). PubMed: 34537242