Recombinant Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1623] to Cytokeratin 17 - Cytoskeleton Marker
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
See all Cytokeratin 17 primary antibodies -
Description
Rabbit monoclonal [EP1623] to Cytokeratin 17 - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431 and HACAT cell lysates Human squamous cervical carcinoma tissue Human tonsil tissue A431 cells HeLa (human cervix adenocarcinoma) cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1623 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab185032)
- Biotin Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab195906)
- Alexa Fluor® 647 Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab196199)
- PE Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab210724)
- Anti-Cytokeratin 17 antibody [EP1623] - BSA and Azide free (ab239986)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
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ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250. |
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
Target
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Function
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. -
Tissue specificity
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level). -
Involvement in disease
Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3872 Human
- Entrez Gene: 16667 Mouse
- Entrez Gene: 287702 Rat
- Omim: 148069 Human
- SwissProt: Q04695 Human
- SwissProt: Q9QWL7 Mouse
- SwissProt: Q6IFU8 Rat
- Unigene: 2785 Human
see all -
Alternative names
- 39.1 antibody
- CK 17 antibody
- CK-17 antibody
see all
Images
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All lanes : Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Cytokeratin 17 with purified ab109725 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 17 with purified ab109725 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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Immunohistochemical analysis of paraffin-embedded human squamous cervical carcinoma tissue using ab109725 at 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109725 at 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of A431 cells using ab109725 at 1/100 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab109725 has been referenced in 25 publications.
- Zhu X et al. HDAC1/2 Control Proliferation and Survival in Adult Epidermis and Pre?Basal Cell Carcinoma through p16 and p53. J Invest Dermatol 142:77-87.e10 (2022). PubMed: 34284046
- Li Z et al. ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation. Nat Commun 13:2011 (2022). PubMed: 35440136
- Chen J et al. KRT17 Promotes the Activation of HSCs via EMT in Liver Fibrosis. J Clin Transl Hepatol 10:207-218 (2022). PubMed: 35528988
- Gurumurthy RK et al. Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection. Nat Protoc 17:1658-1690 (2022). PubMed: 35546639
- Teng YY et al. Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis. Front Bioeng Biotechnol 10:902894 (2022). PubMed: 35832407