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Anti-intestinal FABP antibody [9A9B7B3] (ab109772)

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Overview

  • Product nameAnti-intestinal FABP antibody [9A9B7B3]See all intestinal FABP primary antibodies ...
  • Description
    Mouse monoclonal [9A9B7B3] to intestinal FABP
  • Tested applicationsWB, ELISA, IHC-P, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human intestinal intestinal FABP.

  • Positive controlHuman Small Intestine tissue; LOVO cell lysate, intestinal FABP-transfected HEK293 cell lysate; LOVO and 3T3-L1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab109772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/2000. Predicted molecular weight: 15 kDa.
ELISA ELISA: Use at an assay dependent dilution.
IHC-P IHC-P: 1/100.
Flow Cyt Flow Cyt: 1/200 - 1/400.
ICC/IF ICC/IF: 1/200 - 1/1000.

Target

  • FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificityExpressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P12104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        FABP 2 antibodyFABP2 antibodyFABPI antibody
        FABPI_HUMAN antibodyFatty acid binding protein 2 intestinal antibodyFatty acid binding protein antibodyFatty acid binding protein intestinal antibodyFatty acid-binding protein 2 antibodyFatty acid-binding protein antibodyI FABP antibodyI-FABP antibodyIFABP antibodyintestinal antibodyIntestinal fatty acid binding protein 2 antibodyIntestinal-type fatty acid-binding protein antibodyMGC133132 antibodyOTTHUMP00000163925 antibody
      see all

    Anti-intestinal FABP antibody [9A9B7B3] images

    • Western blot - intestinal FABP antibody [9A9B7B3] (ab109772)
      Western blot - intestinal FABP antibody [9A9B7B3] (ab109772)
      All lanes : Anti-intestinal FABP antibody [9A9B7B3] (ab109772) at 1/500 dilution

      Lane 1 : intestinal FABP-hIgGFc transfected HEK293 cell lysate
      Lane 2 : LOVO cell lysate


      Predicted band size : 15 kDa
    • Flow Cytometry - intestinal FABP antibody [9A9B7B3] (ab109772)
      Flow Cytometry - intestinal FABP antibody [9A9B7B3] (ab109772)
      Flow cytometric analysis of LOVO cells using ab109772 at 1/200 dilution (green) or a negative control (purple).
    • Immunocytochemistry/ Immunofluorescence - intestinal FABP antibody [9A9B7B3] (ab109772)
      Immunocytochemistry/ Immunofluorescence - intestinal FABP antibody [9A9B7B3] (ab109772)
      Immunofluorescence analysis of intestinal FABP in 3T3-L1 cells using ab109772 at 1/200 dilution (green).
      Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - intestinal FABP antibody [9A9B7B3] (ab109772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - intestinal FABP antibody [9A9B7B3] (ab109772)
      Immunohistological staining of Human small intestine, Formalin-Fixed, Paraffin-Embedded tissue using ab109772 at 1/100 dilution.

    References for Anti-intestinal FABP antibody [9A9B7B3] (ab109772)

    ab109772 has not yet been referenced specifically in any publications.

    Publishing research using ab109772 ? Please let us know so that we can cite the reference in this datasheet

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    Product code: 109772
    Inquiry: I would need to know the concentration of the antibody to select the concentration of my isotype

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