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Overview

  • Product nameAnti-Chromogranin A antibodySee all Chromogranin A primary antibodies ...
  • Description
    Goat polyclonal to Chromogranin A
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-QGAKERAHQQKKH, corresponding to internal sequence amino acids 85-97 of Human Chromogranin A (NP_001266.1).

  • Positive controlHuman kidney lysate; Human small intestine and Human pancreas tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab109993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 51 kDa).
IHC-P IHC-P: Use a concentration of 3.75 µg/ml.

Target

  • FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
  • Target information above from: UniProt accession P10645 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • Alternative names
        beta Granin antibodyCgA antibodyCHG A antibody
        Chga antibodyChromogranin A parathyroid secretory protein 1 antibodyChromogranin A precursor antibodyChromograninA antibodyCMGA_HUMAN antibodyER-37 antibodyPancreastatin antibodyParastatin antibodyParathyroid secretory protein 1 antibodyPituitary secretory protein I antibodySP I antibodySP-I antibodySP1 antibodySPI antibodyVasostatin antibodyVasostatin I antibodyVasostatin II antibody
      see all

    Anti-Chromogranin A antibody images

    • Anti-Chromogranin A antibody (ab109993) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
      developed using the ECL technique

      Predicted band size : 51 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour
    • ab109993, at a concentration of 3.75 µg/ml, staining Chromogranin A in formalin fixed, paraffin embedded Human small intestine by Immunohistochemistry.
    • ab109993, at a concentration of 3.75 µg/ml, staining Chromogranin A in formalin fixed, paraffin embedded Human pancreas by Immunohistochemistry.

    References for Anti-Chromogranin A antibody (ab109993)

    ab109993 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"