Fumarase Specific Activity Assay Kit (ab110043)

Overview

• Product nameFumarase Specific Activity Assay KitSee all Fumarase kits ...
• Assay typeEnzyme activity
• Species reactivity
  Reacts with: Rat, Cow, Human
  

  Does not react with

  Mouse
• Product overview

  ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

• Notes

  ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

• Tested applicationsSandwich ELISA more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	96 tests
  100X Coupler	1 x 250µl
  100X Detector Antibody	1 x 130µl
  100X HRP Label	1 x 130µl
  100X Reagent Dye	1 x 250µl
  10X Blocking Buffer	1 x 10ml
  1X Base Buffer	1 x 25ml
  2000X Malate dehydrogenase	1 x 20µl
  200X betaNAD+	1 x 250µl
  200X Fumarate (1M)	1 x 250µl
  96-well microplate (12 strips)	1 unit
  Extraction Buffer	1 x 15ml
  TMB Development Solution	1 x 12ml

• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Other Metabolism Assay

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Fumarase Specific Activity Assay Kit images

• 

  Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
  Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
• 

  Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
  Principle of a Sandwich ELISA.

Protocols

Protocol Booklet