Anti-alpha Actinin antibody [BM 75.2] (ab11008)
Overview
- Product nameAnti-alpha Actinin antibody [BM 75.2]See all alpha Actinin primary antibodies ...
- DescriptionMouse monoclonal [BM 75.2] to alpha Actinin
- SpecificityMonoclonal anti-alpha Actinin specifically stains alpha actinin in cell cultures using indirect immunofluorescent labeling.
- Tested applicationsDouble Immunodiffusion, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Chicken, Human
- Immunogen
Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.
- Positive controlchicken fibroblasts
- General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: Raw Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone numberBM 75.2
- IsotypeIgM
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab11008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Double Immunodiffusion | DID: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 107 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. |
Target
- FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
- Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. - Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
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Database links
- Entrez Gene: 373918 Chicken
- Entrez Gene: 87 Human
- Entrez Gene: 109711 Mouse
- Omim: 102575 Human
- SwissProt: P05094 Chicken
- SwissProt: P12814 Human
- SwissProt: Q7TPR4 Mouse
- Unigene: 509765 Human
- Unigene: 253564 Mouse
- Unigene: 403477 Mouse
see all
Target information above from: UniProt accession
P12814
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- actinin 1 smooth muscle antibodyActinin alpha 1 antibodyactinin, alpha 1 antibody
- ACTN 1 antibodyACTN1 antibodyACTN1_HUMAN antibodyAlpha Actinin 1 antibodyAlpha actinin cytoskeletal isoform antibodyAlpha-actinin cytoskeletal isoform antibodyAlpha-actinin-1 antibodyF actin cross linking protein antibodyF-actin cross-linking protein antibodyFLJ40884 antibodyFLJ54432 antibodyNon muscle alpha actinin 1 antibodyNon-muscle alpha-actinin-1 antibody
see all
Anti-alpha Actinin antibody [BM 75.2] images
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![Western blot - alpha Actinin antibody [BM 75.2] (ab11008)](http://a.abcam.com/ps/datasheet/Images/11/ab11008/ab11008-13787_WB.jpg)
Western blot - alpha Actinin antibody [BM 75.2] (ab11008)Anti-alpha Actinin antibody [BM 75.2] (ab11008) (Human whole cell lysate (MDA MB 231 cells))
Secondary
Sheep anti-mouse HRP
Predicted band size : 107 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)This image is courtesy of an anonymous Abreview
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![Immunocytochemistry/ Immunofluorescence - alpha Actinin antibody [BM 75.2] (ab11008)](http://a.abcam.com/ps/datasheet/images/11/ab11008/alpha-Actinin-Primary-antibodies-ab11008-2.jpg)
Immunocytochemistry/ Immunofluorescence - alpha Actinin antibody [BM 75.2] (ab11008)This image is courtesy of an anonymous Abreviewab11008 staining alpha Actinin in Human fibrosarcoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton. Samples were incubated with 10 µg/ml primary antibody in PBS for 1 hour at 20°C. An Alexa Fluor® -conjugated Donkey monoclonal to mouse IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI.
References for Anti-alpha Actinin antibody [BM 75.2] (ab11008)
This product has been referenced in:
- Averbeck M et al. Switch in syndecan-1 and syndecan-4 expression controls maturation associated dendritic cell motility. Exp Dermatol 16:580-9 (2007). Read more (PubMed: 17576238) »
- Meng X et al. Endometrial regenerative cells: a novel stem cell population. J Transl Med 5:57 (2007). ICC/IF ; Human . Read more (PubMed: 18005405) »
