Overview
- Product nameAnti-15 Lipoxygenase 1 antibody [3D8]See all 15 Lipoxygenase 1 primary antibodies ...
- DescriptionMouse monoclonal [3D8] to 15 Lipoxygenase 1
- Tested applicationsWB, IHC-P, Sandwich ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length Human 15 Lipoxygenase 1 (amino acids 1-663) with a proeprietary tag (AAH29032).
- Positive controlHuman prostate tissue. HeLa nuclear extracts.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Constituent: PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone number3D8
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab110100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 75 kDa. |
| IHC-P | IHC-P: Use a concentration of 5 µg/ml. |
| Sandwich ELISA | sELISA: Use a concentration of 0.003 µg/ml. as a capture antibody. |
Target
- FunctionConverts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid.
- PathwayLipid metabolism; leukotriene D4 biosynthesis.
- Sequence similaritiesBelongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 246 Human
- Omim: 152392 Human
- SwissProt: P16050 Human
- Unigene: 73809 Human
Target information above from: UniProt accession
P16050
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 15 lipoxygenase 1 antibody15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody15 LOX antibody
- 15 LOX 1 antibody15-LOX antibody15LOX 1 antibodyALOX15 antibodyArachidonate 15 lipoxygenase antibodyArachidonate 15-lipoxygenase antibodyArachidonate omega 6 lipoxygenase antibodyArachidonate omega-6 lipoxygenase antibodyLOG15 antibodyLOX15_HUMAN antibody
see all
Anti-15 Lipoxygenase 1 antibody [3D8] images
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![Western blot - 15 Lipoxygenase 1 antibody [3D8] (ab110100)](http://a.abcam.com/ps/datasheet/images/110/ab110100/15-Lipoxygenase-1-Primary-antibodies-ab110100-1.jpg)
Western blot - 15 Lipoxygenase 1 antibody [3D8] (ab110100)Anti-15 Lipoxygenase 1 antibody [3D8] (ab110100) + HeLa nuclear extracts at 50 µg
Predicted band size : 75 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 15 Lipoxygenase 1 antibody [3D8] (ab110100)](http://a.abcam.com/ps/datasheet/images/110/ab110100/15-Lipoxygenase-1-Primary-antibodies-ab110100-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 15 Lipoxygenase 1 antibody [3D8] (ab110100)ab110100 at 5µg/ml staining 15 Lipoxygenase 1 in formalin-fixed, paraffin-embedded Human prostate tissue. -
![Sandwich ELISA - 15 Lipoxygenase 1 antibody [3D8] (ab110100)](http://a.abcam.com/ps/datasheet/images/110/ab110100/15-Lipoxygenase-1-Primary-antibodies-ab110100-3.jpg)
Sandwich ELISA - 15 Lipoxygenase 1 antibody [3D8] (ab110100)Detection limit for ab110100 is approximately 3 ng/ml as a capture antibody.
References for Anti-15 Lipoxygenase 1 antibody [3D8] (ab110100)
ab110100 has not yet been referenced specifically in any publications.
