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MitoSciences (MS850)

Mitochondria Isolation Kit for Tissue (ab110168)

Q&A (33)Specific References (5)

Overview

  • Product nameMitochondria Isolation Kit for TissueSee all Mitochondria Isolation kits ...
  • Sample type
    Tissue
  • Assay typeQuantitative
  • Product overview

    ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

  • Notes

    Tested tissues: brain, liver and heart

  • Tested applicationsFunctional Studies more details

Properties

Applications

Our Abpromise guarantee covers the use of ab110168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Mitochondria Isolation Kit for Tissue images

  • Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
    Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
    Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondra were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Signal strength higher compared to homogenate at respective amounts, see lane 3 and 4.

Protocols

References for Mitochondria Isolation Kit for Tissue (ab110168)

This product has been referenced in:
  • Olga Boyd Nectarine promotes longevity in Drosophila melanogaster Free Radical Biology and Medicine 50:1669-1678 (2011). Read more (PubMed: ) »
  • Olga Boyd Nectarine promotes longevity in Drosophila melanogaster Free Radical Biology and Medicine 50:1669-1678 (2011). Read more (PubMed: ) »

See all 5 Publications for this product

Publishing research using ab110168 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit a Question for ab110168

Displaying 1 - 10 of 33 results for Q&A

10
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Phone call requesting information on how pure the mouce lysate ab110345 is and the kit ab110168



As we discussed over the phone, I have contacted the lab at Mitosciences to get their input into this query. The mouse brain mitochondrial lysate has been prepared using standard mitochondrial isolation procedures. These procedures separate the ...

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Questions for ab110170:



1. Step 3 of the protocol states: Resuspend the cells to 5 mg/ml in Reagent A in a 2-ml microtube. What does the 5 mg/ml refer to?



2. Step 6 of the protocol states: Homogenize the cells...

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Thank you for contacting us at abcam. Please see below under each question the answers.



Questions for ab110170:

1. Step 3 of the protocol states: Resuspend the cells to 5 mg/ml in Reagent A in a 2-ml microtube. What does the...

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Thanks for the reply
can you please tell me what is this starting material in table 1? is this amount of protein or amount of tissue?

Regards:

Thank you for contacting us.

It is the amount of tissue. e.g. from 3-5 grams of liver tissue ˜2-4mg of mitochondrial yield can be expected.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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Product code: 110168
Inquiry: Hi I have one query about this kit, which is as follows: what is the minimum amount of protein required as the starting material for Phosphoenrichment with this kit? and what yield would I obtain after enrichment? Thanks

Thank you for contacting us.

Please check the Table 1 of protocol for answers;

http://www.abcam.com/ps/products/110/ab110168/documents/ab110168%20Mitochondria%20Isolation%20Kit%20for%20Tissue%20booklet%20%20(Website).pdf

I hope ...

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Hi,

I have an enquiry regarding Mitochondria Isolation Kit for Tissue (ab110168). I was wondering if it can be used to isolate mitochondria from frozen skeletal muscle of mouse (as datasheet says only rat brain, heart and liver).

Please ...

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Thank you for your enquiry.

I can confirm that this kit can be used for frozen skeletal muscle of mouse. Please follow the "Heart" protocol. It will be key to mince finely the tissue with a razor before using the dounce, as skeletal muscle also...

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vielen Dank für Ihre schnelle Antwort. Leider hatte ich eine Vorlesung zu
halten, so deshalb konnten Sie mich telefonisch erreichen. Ich habe
erstmal GAPDH und alpha-Tubulin -AK eingesetzt. Aber bei GAPDH bin ich mir
nicht sicher, denn GA...

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Vielen Dank für Ihre Antwort.

Es tut mir sehr leid dass ich in diesem Fall nicht mehr helfen konnte.

Ich habe ein wenig nach vorhandener Literatur gesucht zur Lokalisation von GADPH als auch alpha-Tubulin.

Ich denke Sie hab...

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Name: Mariana Jorge
Product code: 110170

Inquiry: Hi, I am Mariana, one of your distributors here in Brazil. I have a costumer who is interested in this kit, but he has some questions. What is the differences between this kit and the kit A...

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Thank you for contacting us.

Were you referring to ab110168, the Mitochondria Isolation Kit for Tissue? Tje ab11068 is an antibody, so I assume you're talking about this kit.

For ab110170, the cell pellet is frozen in step 2 of the pr...

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Customer kindly contacted us inquiring about sample preparation and storage.

Thank you for contacting us.

Samples may be prepared as purified mitochondria, crudemitochondria. In most cases whole tissue or cell lysates aresuitable but these may require some sample optimization. Mitochondria samples can be prepared bysimp...

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Dear Miss or Mister

I was wondering if this kit to isolate mitochondria (ab110168) works with frozen liver tissue

Best

Thank you for contacting us and your interest in our products.

I can confirm that the Mitochondria Isolation Kit for Tissue (ab110168) can be used with frozen tissue. We would suggest that the liver tissue samples should be flash frozen and store...

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I'm looking for a lysis buffer condition that will solubilize plasma membrane but leave mitochondria intact - any suggestions? With the buffer that you recommend, what happens to nuclei?

Thanks for your enquiry.

We have two kits that are designed to isolate mitochondria:

1. ab110168 - Mitochondrial isolation kit for tissues
2. ab110171 - Mitochondrial isolation kit for cultured cells

You can check the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"