Overview
- Product nameAnti-ARF1 antibody [ARFS 3F1]See all ARF1 primary antibodies ...
- DescriptionMouse monoclonal [ARFS 3F1] to ARF1
- SpecificityThis antibody is specific to class I ARF.
- Tested applicationsIHC-P, ICC/IF, ELISA, WB more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide: SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone numberARFS 3F1
- MyelomaSp2/0-Ag14
- IsotypeIgG2a
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab11038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| ICC/IF | ICC/IF: 1/50. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 22 kDa. |
Target
- FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
- Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
- Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
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Database links
- Entrez Gene: 375 Human
- Omim: 103180 Human
- SwissProt: P84077 Human
- Unigene: 286221 Human
Target information above from: UniProt accession
P84077
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ADP Ribosylation Factor 1 antibodyADP-ribosylation factor 1 antibodyARF 1 antibody
- ARF1 antibodyARF1_HUMAN antibodyDJ133P16.1 antibody
see all
Anti-ARF1 antibody [ARFS 3F1] images
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![Sandwich ELISA - ARF1 antibody [ARFS 3F1] (ab11038)](http://a.abcam.com/ps/datasheet/images/11/ab11038/ARF1-Primary-antibodies-ab11038-4.jpg)
Sandwich ELISA - ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an anonymous Abreviewab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Ab6728 (1/1000) was used as the secondary antibody.
The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight) -
](http://a.abcam.com/ps/datasheet/images/11/ab11038/ARF1-Primary-antibodies-ab11038-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ARF1 antibody [ARFS 3F1](ab11038)IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](http://a.abcam.com/ps/datasheet/images/11/ab11038/ARF1-Primary-antibodies-ab11038-14.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody [ARFS 3F1] (ab11038)This image is courtesy of an Abreview submitted by Armen Petrosyanab11038 staining ARF1 in the Panc-1 cell line from Human pancreatic carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formalddehyde and blocked with 1% Donkey serum in PBST for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% Donkey serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
References for Anti-ARF1 antibody [ARFS 3F1] (ab11038)
This product has been referenced in:
- Lim J et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . Read more (PubMed: 20634316) »
- Tang D et al. Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay. J Biol Chem 283:6085-94 (2008). WB . Read more (PubMed: 18156178) »
