Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
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Overview
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Product name
Anti-Tyrosine Hydroxylase antibody - Neuronal Marker
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mammals -
Immunogen
Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase aa 1 to the C-terminus. Full length SDS denatured protein purified from rat pheochromocytoma.
Database link: P04177 -
Positive control
- WB: PC-12 whole cell lysate. Rat caudate and striatal lysate. IHC-P: Rat brain tissue.
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General notes
ab112 can be used as a marker for dopaminergic and noradrenergic neurons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (16) |
1/750.
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WB | (4) |
1/200. Predicted molecular weight: 60 kDa.
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Notes |
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IHC-P
1/750. |
WB
1/200. Predicted molecular weight: 60 kDa. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 25085 Rat
- SwissProt: P04177 Rat
- Unigene: 11082 Rat
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Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 60 kDa -
Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).
Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.
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ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.
The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.
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ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.
The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
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Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg
Predicted band size: 60 kDa -
Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat striatal lysate at 10 µg
Predicted band size: 60 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (332)
ab112 has been referenced in 332 publications.
- Dong AQ et al. Pramipexole inhibits astrocytic NLRP3 inflammasome activation via Drd3-dependent autophagy in a mouse model of Parkinson's disease. Acta Pharmacol Sin 44:32-43 (2023). PubMed: 35896696
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866
- Bayley JP et al. Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas. PLoS One 17:e0274478 (2022). PubMed: 36178902
- Mazumder S et al. Post-mortem brain histological examination in the substantia nigra and subthalamic nucleus in Parkinson's disease following deep brain stimulation. Front Neurosci 16:948523 (2022). PubMed: 36188463
- Xu J et al. Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. Int J Mol Med 50:N/A (2022). PubMed: 36300203