Overview
- Product nameAldehyde Quantification Assay Kit (Colorimetric)See all Aldehyde kits ...
- Tests1 x 200 test
- Sample typeSaliva, Plasma, Cell culture extracts, Adherent cells, Suspension cells
- Assay typeQuantitative
- Species reactivityReacts with: Human
- Product overview
ab112113 Aldehyde Quantification Assay Kit (Colorimetric) uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. ab112113 provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm. This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.
Visit our FAQs page for tips and troubleshooting. - Notes
ab112113 should be stored desiccated.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 200 tests Aldehyde Standard 1 vial Assay Buffer 1 x 10ml Dilution Buffer 1 x 20ml Yellow Indicator 2 units - Research Areas
Applications
Our Abpromise guarantee covers the use of ab112113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Aldehyde Quantification Assay Kit (Colorimetric) images
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.
Protocols
References for Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)
ab112113 has not yet been referenced specifically in any publications.
