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Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)

Q&A (3)

Overview

  • Product nameCell Viability Assay Kit (Fluorometric - Near InfraRed)See all Cell Viability kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Product overview

    Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. The proprietary far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.

    ab112123 is an effective tool of labeling cells for the investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112123 is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. It comes with reagents sufficient to perform 200 assays in a 96-well plate or 800 assays in a 384-well plate. The kit provides all the essential components with an optimized protocol for microplate reader analysis of cell viability.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112123 should be stored desiccated.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

    • Applications

    Our Abpromise guarantee covers the use of ab112123 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Cell Viability Assay Kit (Fluorometric - Near InfraRed) images

    • Functional Studies - Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)
      Functional Studies - Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)
      CHO-K1 cell number response measured with ab112123.
      CHO-K1 cells at 0 to 50,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Near InfraRed Dye solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 635/670 nm.

    Protocols

    References for Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)

    ab112123 has not yet been referenced specifically in any publications.

    Publishing research using ab112123 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab112123

    Displaying 1 - 3 of 3 results for Q&A

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    Bonjour,

    Merci pour votre offre. Cela nous parait intéressant.

    Nous ne pouvons pas dire quelle offre nous conviendrait le plus étant donné qu’avec le premier kit nous devrons faire des tests pour voir si no...

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    Merci pour votre émail.

    J'attends donc de vous nouvelles pour savoir comment procéder. Veuillez ne pas hésiter à me recontacter, si vous avez besoin de plus de renseignements.

    Je vous souhaite un très...

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    Merci pour vos réponses.
    Je viens de vérifier, nous n’avons pas le FITC pour le vert.
    Est-ce que le Cy3 ou le Texas red sont également appropriés pour l’utilisation du kit near infrared (réf.ab11212...

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    Merci pour votre émail.

    Comme Monsieur Boucher n'est pas au bureau aujourd'hui, je vous réponds.

    Le filtre dufluorophore correspondant du kit ab112123 est le filtre pourCy5. Lesfiltres pour leCy3 et le Texas Red ne sont d...

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    Hi,

    I am using Greiner Thincert Culture Inserts for 6-well plates and A549 cells in my exposure experiments. The goal is to expose the cells at an air/liquid interphase for a certain period and to measure cell viability, oxidative stress marker...

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    Thank you for your enquiry and interest in our products.

    MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the cells...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"