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Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab112139)

Q&A (35)

Overview

  • Product nameLysyl Oxidase Activity Assay Kit (Fluorometric)
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells
  • Assay typeDirect
  • Sensitivity
    40 ng/well
  • Species reactivity
    Reacts with: Pig
    Predicted to work with: all Mammals
  • Product overview

    ab112139 offers a sensitive fluorescent assay for detecting the activity of lysyl oxidase. It utilizes a proprietary LOX substrate that releases hydrogen peroxide detected using our proprietary red fluorescence substrate in HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available assays. It eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell extracts or solutions. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ab112139 should be stored desiccated.

    Please NOTE that a protein standard is not provided with this kit.

  • Tested applicationsFunctional Studies more details

Properties

Applications

Our Abpromise guarantee covers the use of ab112139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Lysyl Oxidase Activity Assay Kit (Fluorometric) images

  • Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric ) (ab112139)
    Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric ) (ab112139)
    ab112139 used to measure Lysyl oxidase dose response on a solid black 96-well with ab112139. As low as 40 ng of lysyl oxidase can be detected with 30 minutes incubation (n=3). The insert shows the low levels of lysyl oxidase detection.

Protocols

References for Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab112139)

ab112139 has not yet been referenced specifically in any publications.

Publishing research using ab112139 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab112139

Displaying 1 - 10 of 35 results for Q&A

10
Votes: Highest First

With this kit, should one use fresh or frozen samples? Also, are tissue extracts OK?


1. Should one use fresh or frozen samples? --Both are OK as long as there is LOX in the sample
2. Are tissue extracts OK?--Yes.


1) In instruction for user on page 9, preparing the serial diluted LOX standards from 0.04ng to 4ug into well from LS1 to LS7 in
duplicate. Is it 0.04ng not 0.04ug?
2) Somebody bought the standard (cat#: 2639-AO) from R&D compamy becaus...

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1) Regarding the standard serial dilutions, you are correct it should be 0.04 ug to 4 ug. This has been updated in our online protocols, but older copies of the protocol may still incorrectly say 0.04 ng. Please make the standards starting with 0.04 ...

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To test losyl oxidase activity(ab112139), when dealing with tissue samples, can I add protease inhibitor?


I contacted the scientists in the lab about this question, and we have not tested the kit with samples containing protease inhibitors, but theoretically the kit should be compatible with protease inhibitors. It should be fine to add these to your sam...

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Product code: 112139
Lot number: Gr115025-2
Inquiry: Hello. We have tried out your LOX activity assay kit on 200 ug protein, also using he RnD LOXstandard you recommend. We now wonder how you would calculate the activity of the enzyme based on the...

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Thank you for contacting us.

The LOX activity is expressed as Fluorescent RFU units. Please check the following publication for reference;

http://www.molecular-cancer.com/content/9/1/206

I hope this information is helpful to you...

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Customer kindly contacted us to inquire about the sensitivity of this product when using colormetric detection.

We do not have precise data for the sensitivity of colorimetric measurement. However, the sensitivity will decrease at least 30-100 times if using colorimetric measurement in theory.

Hello we are trying out your LOX activity kit (ab112139) and we have run out of HRP substrate. In an answer to a customer on your support page (question 15) you say that it is possible to purchase extra HRP substrate for 90 GBP. Is this still possible?

This product was not in our catalogue, however I have contacted the lab and have got their approval for adding the HRP substrate to the catalogue. The ab number is ab155473 and costs €‘125.

I have a very quick question for you.
Does phenol red in tissue culture media interfere with the lysyl oxidase red fluorescence kit?

Thank you for contacting us.

No phenol red in tissue culture media will not interfere with the lysyl oxidase red fluorescence kit, ab112139.

I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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Can I use tissue extract as a sample with this kit? If so, any recommendations for a tissue extraction protocol I can use?

Some optimimization of of your tissue extraction protocol may be required but this kit will detect LOX from tissue extracts.

Product code: 112139
Lot number: GR82615-1
Inquiry: can this kit use tissue homogenated with buffer contain :sucrose,EGTA NaCl,hepes,EDTA and SDS as we use to western blot ,or can we use PBS as we use to ELISA ?

Thank you for contacting us.

This kit will be compatible with lysates as long as they don't contain DTT and any kind of detergent. Your lysates have SDS in these so this kit will not be compatible.

PBS will be fine with this kit.
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Inquiry: What species is your LOX ELISA kit ab112139 for? Thanks

Thank you for contacting us.

Although we have tested the product with Pig LOX protein however the kit will work with a range of species as long as they have the LOX enzyme.

I hope this information is helpful to you. Please do not hesi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"