Rhod-4 Medium Removal No Wash Calcium Assay kit (ab112156)
- Product nameRhod-4 Medium Removal No Wash Calcium Assay kitSee all Calcium kits ...
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time1h 00m
- Species reactivityReacts with: Human
- Product overview
ab112156 provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. Compared to Fluo-8, Rhod-4 is more photostable, making its fluorescence imaging more robust.
Visit our FAQs page for tips and troubleshooting.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit 10X Pluronic® F127 Plus 1 x 1ml HHBS 1 x 9ml Rhod-4 1 vial
Our Abpromise guarantee covers the use of ab112156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Rhod-4 Medium Removal No Wash Calcium Assay kit images
Carbachol Dose Response measured in HEK293 cells using Rhod-4 Medium Removal No Wash Calcium Assay kit and Rhod-2.
HEK293 cells were incubated with 100 μL of the dye-loading solution using ab112156, or 100 μL of Rhod-2 solution (5 μM) for 1 hour at room temperature. Carbachol (25 μL/well) was added to achieve the final indicated concentrations. The EC50 of Rhod-4 is about 0.8 μM, and that for Rhod-2 about 0.7 μM.
References for Rhod-4 Medium Removal No Wash Calcium Assay kit (ab112156)
ab112156 has not yet been referenced specifically in any publications.