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Anti-Albumin antibody (HRP) (ab112891)

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Overview

  • Product nameAnti-Albumin antibody (HRP)See all Albumin primary antibodies ...
  • Description
    Sheep polyclonal to Albumin (HRP)
  • ConjugationHRP
  • Tested applicationsWB, ELISA, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab112891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/30000. Predicted molecular weight: 69 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA ELISA: 1/10000 - 1/100000.
IHC-P IHC-P: 1/200 - 1/500.
ICC ICC: 1/200 - 1/500.

Target

  • FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
        alb antibodyALBU_HUMAN antibodyAlbumin (32 AA) antibody
        Albumin (AA 34) antibodycell growth inhibiting protein 42 antibodyDKFZp779N1935 antibodyGIG20 antibodyGIG42 antibodygrowth-inhibiting protein 20 antibodyOTTHUMP00000220436 antibodyOTTHUMP00000220438 antibodyOTTHUMP00000220439 antibodyPRO0883 antibodyPRO0903 antibodyPRO1341 antibodyPRO1708 antibodyPRO2044 antibodyPRO2619 antibodyPRO2675 antibodySerum albumin antibodyUNQ696/PRO1341 antibody
      see all

    References for Anti-Albumin antibody (HRP) (ab112891)

    ab112891 has not yet been referenced specifically in any publications.

    Publishing research using ab112891 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-Albumin antibody (HRP)

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    Abreviews
    Application Western blot
    Sample Cow Purified protein (bovine serum albumin)
    Loading amount 1.4 µg
    Specification bovine serum albumin
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Nov 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"