Anti-LIF antibody (ab113262)
Key features and details
- Rabbit polyclonal to LIF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-LIF antibody
See all LIF primary antibodies -
Description
Rabbit polyclonal to LIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
A 16 amino acid synthetic peptide corresponding to internal sequence amino acids of Human LIF (NP_002300).
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Positive control
- WB: NIH/3T3 and EL4 cell lysates. Mouse colon and rat lung tissue lysates; ICC/IF: NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab113262 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16878 Mouse
- Entrez Gene: 60584 Rat
- SwissProt: P09056 Mouse
- SwissProt: P17777 Rat
- Unigene: 4964 Mouse
- Unigene: 44379 Rat
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Alternative names
- CDF antibody
- Cholinergic Differentiation Factor antibody
- D factor antibody
see all
Images
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All lanes : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : EL4 (mouse lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml.
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Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : Rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab113262 has been referenced in 18 publications.
- Miyazaki Y et al. Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts. Cancers (Basel) 14:N/A (2022). PubMed: 35326527
- Chen Q et al. Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration. Cell Death Dis 12:417 (2021). PubMed: 33888681
- Wang G et al. Effects of bone marrow mesenchymal stem cells on repair and receptivity of damaged endometrium in rats. J Obstet Gynaecol Res 47:3223-3231 (2021). PubMed: 34184363
- Xie K et al. Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia. Cancer Cell Int 21:627 (2021). PubMed: 34838029
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510