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Overview

  • Product nameEIF3S1 protein
  • Protein descriptionRecombinant fragment, (amino acids 70-258) of Human EIF3S1 with N terminal His tag; 210 amino acids, 24.0 kDa inclusive of tag (NP_003749).
  • Uniprot accessionO75822
  • Molecular weight24kDa inclusive of tags
  • Protein length189 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab113578 is purified using conventional chromatography techniques.
  • Mass spectrometryMALDI-TOF
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.02% Tris HCl, 0.03% DTT, 10% Glycerol, 1.17% Sodium chloride
  • Concentration information loading...
  • SequenceMGSSHHHHHHSSGLVPRGSHMKISEKKKIAEKIKEKERQQ KKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESD LELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKIT QYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCS EKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDG GYVQDYEDFM
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab113578 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    MS MS: Use at an assay dependent concentration.

    Protein info

    • Alternative names
        eIF 3 alphaeIF-3-alphaeIF3 alpha
        eIF3 p35eIF3jEIF3J_HUMANEIF3S 1EIF3S1Eukaryotic translation initiation factor 3 subunit 1Eukaryotic translation initiation factor 3 subunit JEukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD
      see all
  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • Sequence similaritiesBelongs to the eIF-3 subunit J family.
  • Post-translational
    modifications    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession O75822 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    EIF3S1 protein images

    • SDS-PAGE - EIF3S1 protein (ab113578)
      SDS-PAGE - EIF3S1 protein (ab113578)
      15% SDS-PAGE showing ab113578 at approximately 24.0kDa (3µg).

    References for EIF3S1 protein (ab113578)

    ab113578 has not yet been referenced specifically in any publications.

    Publishing research using ab113578 ? Please let us know so that we can cite the reference in this datasheet

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