Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)
Overview
Components
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- Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893)
- ChIP, ChIP/Chip, ICC, ICC/IF, IHC-FoFr, IHC-P, IP, WB
- Rabbit polyclonal (25µg )
- 0.3mg/ml
- Reacts with: Hu, Ms, Rat
- Predicted: Chmp
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- Anti-Histone H2A.X antibody (ab10475)
- ELISA, IHC-P, WB
- Rabbit polyclonal (25µg )
- 0.3mg/ml
- Predicted: Hu, Ms
-
- Anti-RAD54 antibody (ab10705)
- IP, WB
- Rabbit polyclonal (25µg )
- 0.5mg/ml
- Reacts with: Hu
-
- Anti-Rad51 antibody (ab84304)
- WB
- Rabbit polyclonal (25µg )
- 0.3mg/ml
- Reacts with: Hu, Rat
- Predicted: Ms, Chk, Cow, Dog, Rb, Zfsh
Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) images
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Western blot - Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)All lanes : Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) at 1/500 dilution
Lane 1 : Control HeLa cell histone preparation
Lane 2 : Colcemid treated HeLa histone preparation
Lane 3 : Control HeLa cell histone preparation withgamma H2A.X peptide - phospho S139 (ab15645) at 1 µg
Lane 4 : Colcemid treated HeLa histone preparation withgamma H2A.X peptide - phospho S139 (ab15645) at 1 µg
Lane 5 : Control HeLa cell histone preparation withgamma H2A.X peptide - unmodified (ab15646) at 1 µg
Lane 6 : Colcemid treated HeLa histone preparation withgamma H2A.X peptide - unmodified (ab15646) at 1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution -
Western blot - Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)All lanes : Anti-Rad51 antibody (ab84304) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 :Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 minutes -
Western blot - Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)Lane 1 :
Lanes 2 - 6 : Anti-RAD54 antibody (ab10705) at 1/500 dilution
Lane 1 : Marker
Lane 2 : HeLa nuclear cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A432 cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : Hek 293 cell lysate
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) -
Western blot - Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)Lanes 1 & 3 : Anti-Histone H2A.X antibody (ab10475) at 1/500 dilution
Lane 2 : Anti-Histone H2A.X antibody (ab10475) at 1/1000 dilution
Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate
Lane 3 : Calf thymus histone lysate withH2A.X peptide - unmodified (ab15020) at 1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Exposure time : 30 minutes
References for Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)
This product has been referenced in:
- Stimpson KM et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet 6: (2010). Read more (PubMed: 20711355) »
- Zhu H et al. Involvement of Caveolin-1 in repair of DNA damage through both homologous recombination and non-homologous end joining. PLoS One 5:e12055 (2010). Read more (PubMed: 20700465) »
