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Overview

  • Product name3-Nitrotyrosine ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 8 6.9%
    Inter-assay
    Sample n Mean SD CV%
    1 3 13%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    8 ng/ml
  • Range
    8 ng/ml - 1000 ng/ml
  • Product overview

    ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

     

    All species.

  • Notes

    Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

  • Tested applicationsSandwich ELISA more details

Properties

  • Alternative names
      MS969

Applications

Our Abpromise guarantee covers the use of ab116691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA

3-Nitrotyrosine ELISA Kit images

  • Sandwich ELISA - 3-Nitrotyrosine Sandwich ELISA Kit (ab116691)
    Sandwich ELISA - 3-Nitrotyrosine Sandwich ELISA Kit (ab116691)
    Example standard curve.

Protocols

References for 3-Nitrotyrosine ELISA Kit (ab116691)

This product has been referenced in:
  • Tarry-Adkins JL  et al. Poor maternal nutrition followed by accelerated postnatal growth leads to alterations in DNA damage and repair, oxidative and nitrosative stress, and oxidative defense capacity in rat heart. FASEB J 27:379-90 (2013). ELISA ; Rat . Read more (PubMed: 23024373) »

See 1 Publication for this product

Publishing research using ab116691 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit a Question for ab116691

Displaying 1 - 10 of 13 results for Q&A

10
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Can you please confirm if your Elisa kit ab116691 can be used with human samples?



3-nitrotyrosine is a chemical formed in vivo by the reaction of tyrosine with nitrating oxidants, superoxide and nitric acid (http://www.ncbi.nlm.nih.gov/pubmed/1416975) with the chemical formula C9H10N2O5. It's structure does not change between...

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Customer is using this product on mouse lung tissue. They have used this kit in three different ways and found three results.
1) Homogonize samples (pellets), put lysis buffer in the sample, use the supernatant and found no difference between their c...

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Thank you for your enquiry.



Lung is a great sample to run with this assay!


My general recommendation is to homogenize and then sonicate the tissue. Sonicate until the sample is not sticky - DNA released from ruptured nu...

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Which type of ELISA would be the best choice for detecting nitrotyrosine in homogenates of mouse colon: ab116691 (sandwich), or ab113848 (competitive)?

Thank you for contacting us.

The competitive ELISA is considered to be more sensitive than the sandwich ELISA. Both are compatible with your samples

Please do not hesitate to contact us if you need any more advice or information. Read More

I will ask the person in charge of placing orders in our lab to provide the info you required and send it by email. Thank you,

Thank you. I will look for their reply. Please have them reference the number CCExxx in their response and place my name in the subject heading so I can be sure that it is directed to me. Thank you very much for your time and assistance. Please contact...

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Unfortunately the Nitrotyrosine ELISA kit is not working at all with my samples. I tried the appended protocols but sadly I still didn't get any detectable results, eventhough I am positive my samples contain nitrotyrosine as shown by many western blots. ...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer ...

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I am not sure if this helps my situation much. If denaturing the samples or increasing the incubation time won't work, then I don't know what else would. I had already tested the assay with a positive control (3NT BSA purchased from another company) and ...

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Thank you for contacting us. I have contacted the lab regarding the peroxynitrite protocol. They have informed me that they use a peroxinitrite from xxxxxx. The peroxinitrite comes with usage guidelines but I do not know exactly what those are. I ho...

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The customer kindly contacted us as he was having continued problems when using ab116691 3-Nitrotyrosine ELISA Kit on his samples, whole mouse embyos. He has had no signal with these samples. He has followed the tissue lysates protocol with these samples ...

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Thank you for contacting us.



We do not actively denature the nitrated BSA standard, however it is possible that a small amount of degradation may occur during lyophilization. We don’t recommend heating the samples for this ELI...

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The Lysis buffer I use is a commercial one. I use it to prepare samples for Western Blots, which I then aliquot and freeze at -80C

As for my data, the readings for samples are all in the range of -0.001, which shows there's almost no signal ...

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Thank you for your reply.


Our recommendation would be to collect samples using the extraction buffer provided with the kit. Without knowing the recipe of the buffer it is impossible to say whether or not it is compatible. Please let me know ...

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Since this is my first use of this kit I was trying to figure out the best sample dilution to use. My final goal is to measure the amount of 3-nitrotyrosine in mouse whole embryo lysates after drug exposure. Therefore I already homogenized, lysed and quan...

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Thank you for your reply.

Regarding your results - can you provide the formulation of the lysis buffer you are using? As the controls and standards are working, I expect the problem is with the sample preparation. Can you also provide your data? ...

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I ordered the 3-nitrotyrosine ELISA kit (ab116691). I was wondering if its possible to order the 96 well plate strips separately, since I still have plenty of stock for the solutions provided with the kit, but am almost out of wells.
Is it possib...

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Thank you for contacting Abcam regarding ab116691.

Unfortunately we do not sell these components separately. The kits are designed to provide sufficient amounts of reagents for 1 96 well plate, so in order to acquire additional strips, you would ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"