EpiSeeker Fast DNA modification Kit (ab117127)
- Product nameEpiSeeker Fast DNA modification KitSee all DNA modification kits ...
- Tests1 x 50 test
- Assay time0h 30m
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Plants, all Mammals
- Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
EpiSeeker Fast DNA modification Kit (ab117127) allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step - but with ab117127, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. ab117127 is suitable for MS-PCR, real time MS-PCR, bisulfite sequencing, pyrosequencing and methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
Store all components at room temperature (15-22°C) away from light.
The kit can be stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 50 tests EBM1 (Conversion Mix Solution) 1 x 6ml EBM2 (Capture Solution) 1 x 15ml EBM3 (Desulphonation Solution) 1 x 60µl EBM4 (Elution Solution) 1 x 1ml EBM5 (Conversion Enhancer) 1 x 5 vials
- Research Areas
Our Abpromise guarantee covers the use of ab117127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS. Minimum DNA amount: 200pg-1µg/ reaction. Optimal DNA amount: 50-200ng/ reaction|
EpiSeeker Fast DNA modification Kit images
Complete Cytosine Conversion: 200 ng of genomic DNA isolated from 3 cancer cell lines was treated with ab117127. Next, the unconverted and converted DNA in each treated sample were determined using unconverted DNA-specific and converted DNA-specific primers (ß-actin, 110 bps), respectively. A: real time PCR; B: end-point PCR. The ab117127 treated DNA was completely converted, and no unconverted DNA in the treated samples was determined after 45 cycles.
Effective DNA Protection: Fully methylated human genomic DNA at various amounts (0.2 ng-200 ng) were converted using the ab117127. 1 µl of 20 µl eluate was used for real time qPCR and a pair of primers was used to amplify converted DNA. As little as 0.2 ng DNA is sufficient for bisulfite conversion using the ab117127. A: real time PCR; B: starting DNA amount-CT value curve.
References for EpiSeeker Fast DNA modification Kit (ab117127)
ab117127 has not yet been referenced specifically in any publications.