Anti-alpha Fodrin antibody [D8B7] (ab11755)

Overview

• Product nameAnti-alpha Fodrin antibody [D8B7]See all Alpha Fodrin primary antibodies ...
• Description
  Mouse monoclonal [D8B7] to alpha Fodrin
• SpecificityClone D8B7 is developed against the alpha II spectrin SH3 domain (Xu et al., 2001). Clone D8B7 is a specific marker for human, rat and mouse alpha Fodrin or alpha II spectrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000). It does not crossreact with alpha I (erythroid) spectrin SH3 domain.
• Tested applicationsICC/IF, IP, IHC-P, ELISA, WB, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human, African Green Monkey, Chinese Hamster
  
• Immunogen

  Recombinant alpha Fodrin (non-erythroid spectrin) SH3 domain (Human).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium azide
  Constituent: PBS
• PurityTissue culture supernatant
• Purification notesTissue Cultue Supernatant (Ammonium sulfate precipitated & dialyzed).
• Clonality Monoclonal
• Clone numberD8B7
• IsotypeIgG2b
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Crosslinking

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Neuronal

Applications

Target

• FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
• Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
• Sequence similaritiesBelongs to the spectrin family.
  Contains 3 EF-hand domains.
  Contains 1 SH3 domain.
  Contains 23 spectrin repeats.
• Post-translational
  modificationsPhosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
• Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.

Target information above from: UniProt accession Q13813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6709 Human
  • Entrez Gene: 64159 Rat
  • Omim: 182810 Human
  • SwissProt: Q13813 Human
  • SwissProt: P16546 Mouse
  • SwissProt: P16086 Rat
  • Unigene: 372331 Human
  • Unigene: 204969 Mouse

  • Unigene: 5812 Rat

  see all

• Alternative names
  (ALPHA)II-SPECTRIN antibodyAlpha II Spectrin antibodyAlpha-II spectrin antibody

  brain antibodyEIEE5 antibodyFLJ17738 antibodyFLJ44613 antibodyFodrin alpha chain antibodyFodrin, alpha antibodyNEAS antibodyNon erythrocytic spectrin alpha antibodynon-erythroid alpha chain antibodySPECA antibodySpectrin alpha chain antibodySpectrin alpha chain brain antibodySpectrin alpha non erythrocytic 1 antibodySpectrin antibodySpectrin non erythroid alpha chain antibodySpectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibodySpectrin, nonerythroid, alpha subunit antibodySpna2 antibodySPTA 2 antibodySPTA2 antibodySPTA2_HUMAN antibodySPTAN 1 antibodySPTAN1 antibody

  see all

Anti-alpha Fodrin antibody [D8B7] images

• 

  Immunocytochemistry/ Immunofluorescence - alpha Fodrin antibody [D8B7] (ab11755)
  IF using ab11755.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Fodrin antibody [D8B7] (ab11755)This image is courtesy of an anonymous Abreview
  ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

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• 

  Western blot - alpha Fodrin antibody [D8B7] (ab11755)
  Anti-alpha Fodrin antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg
  
  Secondary
  HRP conjugated goat anti-mouse at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 297 kDa
  Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes

  This image is courtesy of an anonymous Abreview

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