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Overview

  • Product nameAnti-HA tag antibody (HRP)See all HA tag primary antibodies ...
  • Description
    Rabbit polyclonal to HA tag (HRP)
  • ConjugationHRP
  • SpecificityIn a direct ELISA against 1ug of HA peptide coupled to BSA as sample, conjugate diluted to 1/15,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • Tested applicationsELISA, WB more details
  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage buffer50 mM HEPES pH 7.0, 0.1 M NaCl with 0.2% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use a concentration of 0.05 - 0.5 µg/ml.
WB WB: Use a concentration of 0.2 - 1 µg/ml.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
      HA epitope tag antibodyhemagglutinin antibody

References for Anti-HA tag antibody (HRP) (ab1188)

This product has been referenced in:
  • Uriarte SM  et al. Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst. J Immunol 187:391-400 (2011). WB . Read more (PubMed: 21642540) »

See 1 Publication for this product

Publishing research using ab1188 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab1188 Submit a Question for ab1188

Displaying 1 - 6 of 6 results for Abreviews and Q&A

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Dear Mister or Madam,
We recently ordered you 2 antibodies:

- Anti-HA tag antibody (HRP) (ab1188) (lot: GR27817-2)

- Mouse anti-Human IgG Fc (HRP) (ab99765) (lot: GR73759-1)

However after few experiments the Anti-HA t...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Thank yo...

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Western blot abreview for Anti-HA tag antibody (HRP)

 Poor
Abreviews
Application Western blot
Sample Human Cell lysate - other (human)
Loading amount 20 µg
Specification human
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Apr 17 2007

so I loaded at least 50 ug of total protein onto the gel. ran and wet-transferred to the PVDF membrane. then incubate with the primary Ab at 4degC overnight. The primary Ab was used at a 1:1000 dilution in 5% milk in 1X TBS. I tried 1:500 dilution a...

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Thank you for your email and patience. I have tried contacting the originator of this antibody but unfortunately have not heard back. I,m not sure why the antibody is not recognizing the HA tag in your other samples. What was your order number? If you o...

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I've purchased this antibody (ab1188) and tried a Western blot... I've tried different dilutions and buffers. However, the antibody is not recognizing my HA-tagged protein in mammalian cells, only in E. coli cells. Can I return my antibody for a refun...

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Could we get a detailed protocol from you, please? We have some general questions, the answers to which will enable us to investigate this matter as quickly as possible. Also, please include the lot number of ab1188 that you received (it is located o...

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I am looking to stain for an HA-tagged wingless protein in drosophila. I will be staining whole embryos and want a primary grown in Rabbit. My secondary is an anti-rabbit Cy5. I am worried about varying concentrations between batches so I think I need ...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody. If you have any more questions, do not he...

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I would like to know if this antibody HA works also in the native gel for the normal western blots, is there any special protocol? Is there any special dilution ratio for it?

There is no special protocol necessary for using our antibody in this system. As long as the HA tag is exposed, the antibody will be useful. We recommend a starting dilution of 1:1000.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"