IFN-omega Human ELISA kit (with color giving dyes) (ab119529)
Overview
- Product nameIFN-omega Human ELISA kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 6.8% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity1.5 pg/ml
- Range4.7 pg/ml - 300 pg/ml
- Recovery
91 %
- Species reactivityReacts with: Human
- Product overview
ab119529 IFN-omega Human ELISA kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human IFN-omega. ab119529 is for research use only and is not for diagnostic or therapeutic procedures.
In the Human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of Human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-human IFN-omega monoclonal antibody 1 x 200µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human IFN-omega Standard 2 x 50µl Monoclonal anti-human IFN-omega-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml - Research Areas
Target information above from: UniProt accession
P05000
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- IFNW1IFNW1_HUMANInterferon alpha-II-1Interferon omega-1
- Entrez Gene: 3467 Human
- Omim: 147553 Human
- SwissProt: P05000 Human
- Unigene: 73010 Human
Applications
Our Abpromise guarantee covers the use of ab119529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
IFN-omega Human ELISA kit (with color giving dyes) images
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Sandwich ELISA - IFN-omega Human ELISA kit (with color giving dyes) (ab119529)Representative standard curve using ab119529.
Protocols
References for IFN-omega Human ELISA kit (with color giving dyes) (ab119529)
ab119529 has not yet been referenced specifically in any publications.
