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Overview

  • Product nameLaminin Mouse ELISA KitSee all Laminin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's Laminin Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Laminin specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Laminin amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Involvement in diseaseDefects in LAMB2 are the cause of Pierson syndrome (PIERSS) [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome.
    Defects in LAMB2 are a cause of congenital nephrotic syndrome (CONPHS) [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
  • Sequence similaritiesContains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.

    • Target information above from: UniProt accession P55268 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Lamb2LAMB2_HUMANLAMC1
        LAMC2LAMC3laminin ALaminin BLaminin B1s chainLaminin gamma 1Laminin gamma 2Laminin gamma 3Laminin subunit beta-2Laminin-11 subunit betaLaminin-14 subunit betaLaminin-15 subunit betaLaminin-3 subunit betaLaminin-4 subunit betaLaminin-7 subunit betaLaminin-9 subunit betaOCCMS-LAM betaS-laminin subunit beta
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119572 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Laminin Mouse ELISA Kit images

      • Sandwich ELISA - Laminin Mouse ELISA kit (ab119572)
        Sandwich ELISA - Laminin Mouse ELISA kit (ab119572)
        Representative standard curve using ab119572.

      Protocols

      References for Laminin Mouse ELISA Kit (ab119572)

      ab119572 has not yet been referenced specifically in any publications.

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