Overview
- Product nameACE Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 5 pg/ml
- Range156 pg/ml - 10000 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119577 ACE Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human ACE specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ACE amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-human ACE antibody 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-human ACE antibody 1 x 130µl human ACE standard 2 x 10ng Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml - Research Areas
Defects in ACE are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3) [MIM:612624]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention.
Target information above from: UniProt accession
P12821
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACEACE_HUMANACE1
- Angiotensin converting enzyme somatic isoformAngiotensin I converting enzyme (peptidyl dipeptidase A) 1Angiotensin-converting enzymeCarboxycathepsinCD143CD143 antigenDCPDCP1Dipeptidyl carboxypeptidase 1Dipeptidyl carboxypeptidase IKininase IIMVCD3Peptidase Psoluble formTesticular ECA
see all
- Entrez Gene: 1636 Human
- Omim: 106180 Human
- SwissProt: P12821 Human
- Unigene: 298469 Human
Applications
Our Abpromise guarantee covers the use of ab119577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
ACE Human ELISA Kit images
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Sandwich ELISA - ACE Human ELISA Kit (ab119577)Representative standard curve using ab119577.
Protocols
References for ACE Human ELISA Kit (ab119577)
ab119577 has not yet been referenced specifically in any publications.
