You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product namePhosphoTracer S6 RP Total ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    1 µg/ml
  • Range
    1 µg/ml - 250 µg/ml
  • Assay time
    1h 15m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer S6 RP assay kits detect endogenous levels of S6 RP (GenBank Accession NP_001001) in cellular lysates. Total RPS6 assay kits detect RPS6 irrespective of phosphorylation status.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
  • Sequence similaritiesBelongs to the ribosomal protein S6e family.
  • Post-translational
    modifications    Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.

    • Target information above from: UniProt accession P62753 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        40S ribosomal protein S6Phosphoprotein NP33Ribosomal protein S6
        rps6RS6_HUMANS6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119645 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA. Tested in MCF7, PC3, NIH3T3, A549, HEK293, HeLa, U2OS.

      PhosphoTracer S6 RP Total ELISA Kit images

      • Sandwich ELISA - PhosphoTracer S6 RP Total ELISA kit (ab119645)
        Sandwich ELISA - PhosphoTracer S6 RP Total ELISA kit (ab119645)
        Using the S6 RP assay kits or Western blot, S6 RP phosphorylation at Ser240/244 is detected in insulin-treated MCF7 cells (+), compared with MCF7 cells treated with a combination of rapamycin, UCN-01 and U0126 (-), whereas no change in total S6 RP levels was observed.
      • Sandwich ELISA - PhosphoTracer S6 RP Total ELISA Kit (ab119645)
        Sandwich ELISA - PhosphoTracer S6 RP Total ELISA Kit (ab119645)
        PhosphoTracer S6 RP Total ELISA Kit (ab119645) used in Sandwich ELISA. The results show that S6 RP is readily detectable in several cell lines.
      • Sandwich ELISA - PhosphoTracer S6 RP Total ELISA kit (ab119645)
        Sandwich ELISA - PhosphoTracer S6 RP Total ELISA kit (ab119645)
        MCF7 cells were seeded at 40K cells/well in medium containing 10% FBS in a 96 well microplate, and cultured overnight. The following day the culture medium was removed, and the cells were treated with various concentrations of rapamycin diluted in serum-free medium for 60 mins. The medium was removed from the wells, and the cells were lysed with 120 µL/well Lysis Mix, with shaking for 10 min. 50 µL of lysate was transferred to replicate wells of a PhosphoTracer assay plate, and Antibody Mix specific for either phospho S6 RP or total S6 RP (50 µL/well) was added to the lysates. The plates were incubated for 1 hr at room temp, with shaking. Plates were washed and Substrate Mix was added. The plates were covered in foil and incubated for 10 min with shaking. Signal in the wells was determined using a plate reader.

      Protocols

      References for PhosphoTracer S6 RP Total ELISA Kit (ab119645)

      ab119645 has not yet been referenced specifically in any publications.

      Publishing research using ab119645 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119645

      There are currently no Abreviews for ab119645.
      Please use the link above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"