Overview
Properties
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Synthetic
Applications
Our Abpromise guarantee covers the use of ab120647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS: Use at an assay dependent concentration. |
Honokiol images
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Functional Studies - Honokiol (ab120647)MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol(ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79)(ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Functional Studies - Honokiol (ab120647)MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol(ab120647). Increased expression of AMPKα 1 + AMPKα 2 (phospho T172) (ab23875) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab23875 at 1/1000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
References for Honokiol (ab120647)
This product has been referenced in:
- Arora S et al. Honokiol arrests cell cycle, induces apoptosis, and potentiates the cytotoxic effect of gemcitabine in human pancreatic cancer cells. PLoS One 6:e21573 (2011). Read more (PubMed: 21720559) »
- Munroe ME et al. Anti-inflammatory effects of the neurotransmitter agonist Honokiol in a mouse model of allergic asthma. J Immunol 185:5586-97 (2010). Read more (PubMed: 20889543) »
- Fried LE & Arbiser JL Honokiol, a multifunctional antiangiogenic and antitumor agent. Antioxid Redox Signal 11:1139-48 (2009). Read more (PubMed: 19203212) »
