SIRT1 Human ELISA Kit (ab123457)
- Product nameSIRT1 Human ELISA KitSee all SIRT1 kits ...
Intra-assay Sample n Mean SD CV% Overall 8 7%
- Tests1 x 96 well plate
- Sample typeTissue Extracts, Cell Lysate
- Assay typeSandwich
- Sensitivity8 ng/ml
Sample specific recovery Sample type Average % Range Serum 106 98% - 112%
- Species reactivityReacts with: Human
Does not react withMouse, Rat
- Product overview
ab123457 SIRT1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SIRT1 in cell and tissue lysates. The assay employs an antibody specific for Human SIRT1 protein coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- SIRT1 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– human. Rat, mouse are not suitable with this kit, other species are untested.
Typical working ranges:
Tissue extract (e.g. Heart): None detected
Whole cultured cell extract: 0.060 - 1 mg/mL
Human SIRT1 standard: 0.008 - 0.5 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X SIRT1 Detector Antibody 1 x 1ml 20X Buffer 1 x 20ml Extraction Buffer 1 x 15ml Human SIRT1 Standard 1 x 1µg SIRT1 Microplate 1 unit TMB Development Solution 1 x 12ml
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
Contains 1 deacetylase sirtuin-type domain.
modificationsS-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
- hSIR2hSIRT1NAD-dependent deacetylase sirtuin-1
- Regulatory protein SIR2 homolog 1SIR2-like protein 1SIR2L1Sirt1SIRT1_HUMAN
Our Abpromise guarantee covers the use of ab123457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SIRT1 Human ELISA Kit images
Example standard curve. Serially diluted recombinant SIRT1 in the working range of the assay.
Example of serially titrated HeLa extract in the assay working range.
The expression level of SIRT1 varies by cell type. Four cell types are analyzed by SIRT1 expression level relative to the Human HepG2 cell line. This relative relationship is confirmed by Western blotting using antibody ab13533.
The SIRT1 detector antibody (ab110304) in this ELISA is available as standalone antibody. In this image Immunocytochemistry of the antibody (ab110304) from this ELISA used to stain Human HDFn cells.
References for SIRT1 Human ELISA Kit (ab123457)
ab123457 has not yet been referenced specifically in any publications.