Overview
- Product nameAnti-DBF4 antibody [EPR5635]See all DBF4 primary antibodies ...
- DescriptionRabbit monoclonal [EPR5635] to DBF4
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Does not react with
Mouse, Rat - Immunogen
Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).
- Positive controlPC-3, Jurkat, SW480, and HepG2 cell lysates.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 2.49 x 10-10 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5635
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab124707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/10000 - 1/50000. Predicted molecular weight: 77 kDa. |
Target
- FunctionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
- Tissue specificityHighly expressed in testis and thymus. Expressed also in most cancer cells lines.
- Sequence similaritiesContains 2 BRCT domains.
Contains 1 DBF4-type zinc finger. - Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationNucleus.
-
Database links
- Entrez Gene: 10926 Human
- Entrez Gene: 10926 Human
- Entrez Gene: 10926 Human
- Entrez Gene: 10926 Human
- Omim: 604281 Human
- SwissProt: Q9UBU7 Human
- SwissProt: Q9UBU7 Human
- SwissProt: Q9UBU7 Human
- SwissProt: Q9UBU7 Human
- Unigene: 485380 Human
see all
Target information above from: UniProt accession
Q9UBU7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Activator of S phase kinase antibodyASK antibodyChiffon homolog A antibody
- DBF4 antibodyDBF4 type zinc finger containing protein 1 antibodyDBF4-type zinc finger-containing protein 1 antibodyDBF4A antibodyDBF4A_HUMAN antibodyProtein DBF4 homolog A antibodyZDBF1 antibody
see all
Anti-DBF4 antibody [EPR5635] images
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![Western blot - Anti-DBF4 antibody [EPR5635] (ab124707)](http://a.abcam.com/ps/datasheet/images/124/ab124707/DBF4-Primary-antibodies-ab124707-1.jpg)
Western blot - Anti-DBF4 antibody [EPR5635] (ab124707)All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution
Lane 1 : PC-3 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size : 77 kDa -
](http://a.abcam.com/ps/datasheet/images/124/ab124707/DBF4-Primary-antibodies-ab124707-2.jpg)
Other-Anti-DBF4 antibody [EPR5635](ab124707)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-DBF4 antibody [EPR5635] (ab124707)
ab124707 has not yet been referenced specifically in any publications.
