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Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

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Overview

  • Product nameAnti-GAPDH antibody [GA1R] - Loading ControlSee all GAPDH primary antibodies ...
  • Description
    Mouse monoclonal [GA1R] to GAPDH - Loading Control
  • Specificityab125247 recognizes native and denatured forms of GAPDH.
  • Tested applicationsWB, Dot Blot, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Other
    Predicted to work with: a wide range of other species
  • Immunogen

    Recombinant GAPDH.

  • Positive controlHuman, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • General notesStore at 4°C (add 0.05% NaN3) for several days to weeks.

Applications

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot Blot Dot: Use at an assay dependent concentration.
ELISA ELISA: Use at an assay dependent concentration.
ICC/IF ICC/IF: Use at an assay dependent concentration.

Target

  • Alternative names
      Aging associated gene 9 protein antibodyG3P_HUMAN antibodyG3PD antibody
      G3PDH antibodyGAPD antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody [GA1R] - Loading Control images

  • Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    developed using the ECL technique

    Predicted band size : 36 kDa
  • Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    developed using the ECL technique

    Predicted band size : 36 kDa

References for Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

ab125247 has not yet been referenced specifically in any publications.

Publishing research using ab125247 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit a Question for ab125247

Displaying 1 - 10 of 11 results for Q&A

10
Votes: Highest First

Hi

Thank you very much for your reply.

I have tried the replacement antibody and we got it to work in the first shot using a slightly modified protocol from the one that didn’t work initially: 1:500 dilution and overnight incubatio...

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Thank you for that feedback. I am pleased to hear that the replacement antibody has proved successful for you.

I do not completely understand why at a dilution of 1/500 you would only be able to perform two western blots. There should be enough f...

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Thank you for sending a replacement antibody. We’ll be running some tests once we have received a more detailed reference protocol from you and can hopefully get it working.

Yes, the valproic acid has been used in an assay as a reference co...

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I am very sorry for the delay in getting back to you in regards to the Western blotting protocol used with the Anti-GAPDH antibody [GA1R] ab125247.

I can now confirm that the following conditions were used to obtain the image presented on the dat...

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hello, we would need to use a gdph antibody for fly brain. do you know if that one will work.

Thank you very much for contacting Abcam.

To our knowledge, ab125247 has not been tested in Fly/Drosophila tissue. Therefore, I can offer you our AbTrial program where you can now use our products in an untested application or species without fin...

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Dear xxxxx
Thank you for sending a replacement antibody. We’ll be running some tests once we have received a more detailed reference protocol from you and can hopefully get it working.

Yes, the valproic acid has been used in an assay...

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Thank you for getting back to me.

I think the protocol you have been using should be suitable. I would however make sure that the samples are sufficiently lysed, using RIPA buffer and loading onto the gel using Laminae buffer with reducing agent...

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In reply to your questitions:

Yes the RIPA buffer gave a color change of the Bradford reagent. But we do not wish to use the RIPA buffer as it contains detergent, which we want to avoid because it might extract some of our membrane protein. The...

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Thank you for your reply.

I would be concerned that you are getting a colour change in your Bradford reagent without any sample present. This would suggest that there is a contamination in your buffers. I would make new sterile ones to make sure...

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Order date: 17th of july

I’m overexpressing in sf9 cells yes and detecting the poorly membrane protein by the FLAG tag. This protein is approximately 56 kDa but ruuning on the gels close to 40 kDa as it is a membrane protein.

I...

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Thank you for your reply.

I think it is very important to check how much protein is being loaded to keep it within a manageable range, loading too much can lead to aggregation of proteins and high background whilst loading too little can obvious...

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Hi
I’ll have to get back to you with the order number as our technician has gone for the day, but is probably ordered in june or july. The first experiments have been done in late july.

The antibody for the membrane protein is an ant...

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Thank you for confirming that information.

It would be helpful to have the order number if possible. I have found one order of the 17th of July delivered to the University of Copenhagen, Faculty of Health Sciences. Is this your order?

...

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Product code: 125247
Lot number: GR80148-4

Inquiry: The problem seems to be unspecific binding of some sort/ no specific binding at all. We ant to detect GAPDH in sf9 cells.The exact protein concentration is unknown, but we are able to blo...

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Thank you for sharing this information.

I am currently consulting with the source of this antibody to try to understand why the results have not been as expected with this antibody.

In order to investigate the situation further, could ...

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  Hope all is well at your end. Well, I would like to use the abreview offer that is specified in your following email. I have just finished my abreview in abcam website that you can see through clicking the following link. Now, I would like to use the o...

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Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code, yo...

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I would like to thank you for sending a replacement for GAPDH. The one that you sent is ab125247 and it works amazing on yeast cell lysate. Thanks again for your exceptional co-operation.

Thank you for your response. It was my pleasure to help and I am glad that the replacement has worked for you.

Please let us know if there is anything else that we at Abcam might do to help you to meet your research gols.



Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"