Overview
Product nameUbiquitin protein (Human)See all Ubiquitin proteins and peptides ...
Protein descriptionRecombinant full length Human wild type Ubiquitin, 8.6kDa. (P0CG47)
Uniprot accessionP0CG47
Molecular weight9kDa
Protein length229 amino acids
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesPurity is > 95% by RP-HPLC.
FormLiquid
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.61% Tris, 10% Glycerol, 0.88% Sodium chloride
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab125831
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
| MS |
MS: Use at an assay dependent dilution. |
| HPLC |
HPLC: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab137025.
Protein info
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Alternative names
FLJ25987MGC8385Polyubiquitin B
RPS 27ARPS27AUBA 52UBA 80UBA52UBA80UBBUBB_HUMANUBCUBCEP 1UBCEP 2UBCEP1UBCEP2UbiquitinUbiquitin B
see all
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Similarity: Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
References for Ubiquitin protein (Human) (ab125831)
ab125831
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
