Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

Overview

• Product nameAnti-Kaiso antibody [6F / 6F8] - ChIP GradeSee all Kaiso primary antibodies ...
• Description
  Mouse monoclonal [6F / 6F8] to Kaiso - ChIP Grade
• Tested applicationsFlow Cyt, IHC-P, ChIP, ICC/IF, WB, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human
  
• Immunogen

  His-tagged Kaiso fusion protein (Human) lacking the zinc finger region.

• Positive controlCSML0 cells (IP then Western). In Flow Cytometry, this antibody gave a positive signal in HeLa cells.
• General notes

  We can conjugate this antibody to FITC for you (please see ab150256 for details).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number6F / 6F8
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • Methylated DNA

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionTranscriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
• Tissue specificityExpressed in vascular endothelium.
• Sequence similaritiesContains 1 BTB (POZ) domain.
  Contains 3 C2H2-type zinc fingers.
• Cellular localizationNucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.

Target information above from: UniProt accession Q86T24 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10009 Human
  • Entrez Gene: 10009 Human
  • Entrez Gene: 10009 Human
  • Entrez Gene: 56805 Mouse
  • Entrez Gene: 501506 Rat
  • Omim: 300329 Human
  • SwissProt: Q86T24 Human
  • SwissProt: Q86T24 Human

  • SwissProt: Q86T24 Human
  • SwissProt: Q8BN78 Mouse
  • Unigene: 143604 Human
  • Unigene: 391248 Mouse
  • Unigene: 169266 Rat

  see all

• Alternative names
  Kaiso antibodyKaiso protein antibodyKaiso transcription factor antibody

  KAISO_HUMAN antibodyTranscriptional regulator Kaiso antibodyZBTB 33 antibodyZBTB33 antibodyZinc finger and BTB domain containing 33 antibodyZinc finger and BTB domain containing 33 antibodyZinc finger and BTB domain-containing protein 33 antibodyZinc finger transcription factor Kaiso antibodyZNF 348 antibodyZNF Kaiso antibodyZNF348 antibody

  see all

Anti-Kaiso antibody [6F / 6F8] - ChIP Grade images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
  Immunofluorescence using clone 6F / 6F8 on C2C12 cells.
• 

  Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
  
  
  Predicted band size : 80 kDa
  
  
  Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
• 

  Flow Cytometry-Anti-Kaiso antibody [6F / 6F8] - ChIP Grade(ab12723)
  Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.
  Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.
• 

  Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
  
  
  Predicted band size : 80 kDa
  

  Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.