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Overview

  • Product nameUbe2L3 protein (His tag)See all Ube2L3 proteins and peptides ...
  • Protein descriptionRecombinant full length Human Ube2L3 (amino acids 1-154), with N terminal His tag; 17.9kDa without tag (calculated). (UniProt ID = P68036).
  • Uniprot accessionP68036
  • Molecular weight17.900kDa
  • Protein length154 amino acids
  • Expression hostE. coli

    • Properties

  • Purification notesab127411 is purified by Nickel IMAC chromatography using the N-terminal His6 tag. Purity is greater than 95% by HPLC.
  • E.C. Number6.3.2.19
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituents: 0.15% DTT, 0.61% Tris, 10% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Additional notesThe quantity of 75µl corresponds to 3 nmol of protein.
  • Sequence notesMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV PDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNID EKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHP LRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab127411 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    HPLC HPLC: Use at an assay dependent concentration.
  • Application notesThis peptide can be used with studies using ab37913,ab66944 and ab155137.

    • Protein info

    • Alternative names
        E2 F1L UBCL-UBC
        UB2L3_HUMANUBCE7UbcH7UbcM4Ube2l3Ubiquitin carrier protein L3Ubiquitin conjugating enzyme E2 L3Ubiquitin protein ligase L3Ubiquitin-conjugating enzyme E2 L3Ubiquitin-conjugating enzyme E2-F1Ubiquitin-protein ligase L3
      see all
  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificityUbiquitous, with highest expression in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.

    • Target information above from: UniProt accession P68036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Ube2L3 protein (His tag) images

    • HPLC - Ube2L3 protein (His tag) (ab127411)
      HPLC - Ube2L3 protein (His tag) (ab127411)
      HPLC analysis of ab127411

    References for Ube2L3 protein (His tag) (ab127411)

    ab127411 has not yet been referenced specifically in any publications.

    Publishing research using ab127411 ? Please let us know so that we can cite the reference in this datasheet

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