Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab13537)

Overview

• Product nameAnti-Dnmt1 antibody [60B1220.1] - ChIP GradeSee all Dnmt1 primary antibodies ...
• Description
  Mouse monoclonal [60B1220.1] to Dnmt1 - ChIP Grade
• SpecificityThis antibody detects a ~180 kDa protein, corresponding to the apparent molecular mass of Dnmt1 on SDS-PAGE immunoblots in samples of human and mouse origin. Immunogen itself has been shown to be toxic.
• Tested applicationsIHC-P, IHC-Fr, ChIP, Flow Cyt, WB, IP more details
• Species reactivity
  Reacts with: Mouse, Human, Zebrafish
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide: EKDDREDKENAFKR, corresponding to amino acids 637-650 of Human Dnmt1

  Run BLAST with Run BLAST with
• Positive controlHuman kidney (IHC), mouse ES or NIH 3T3 cell lysate (WB)
• General notesFor maximum product recovery, centrifuge the product vial before removing cap. Shipped at on gel packs.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 0.05% BSA, PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is affinity purified.
• Clonality Monoclonal
• Clone number60B1220.1
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • DNMTs

Applications

Target

• FunctionMethylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
• Tissue specificityUbiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
• Sequence similaritiesBelongs to the C5-methyltransferase family.
  Contains 2 BAH domains.
  Contains 1 CXXC-type zinc finger.
• DomainThe N-terminal part is required for homodimerization and acts as a regulatory domain.
• Post-translational
  modificationsSumoylated; sumoylation increases activity.
• Cellular localizationNucleus.

Target information above from: UniProt accession P26358 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281119 Cow
  • Entrez Gene: 1786 Human
  • Entrez Gene: 13433 Mouse
  • Entrez Gene: 30430 Zebrafish
  • Omim: 126375 Human
  • SwissProt: Q24K09 Cow
  • SwissProt: P26358 Human
  • SwissProt: P13864 Mouse

  • Unigene: 202672 Human
  • Unigene: 128580 Mouse
  • Unigene: 25162 Zebrafish

  see all

• Alternative names
  AIM antibodyCXXC finger protein 9 antibodyCXXC-type zinc finger protein 9 antibody

  CXXC9 antibodyDNA (cytosine 5 ) methyltransferase 1 antibodyDNA (cytosine-5)-methyltransferase 1 antibodyDNA methyltransferase 1 antibodyDNA methyltransferase HsaI antibodyDNA methyltransferase M.HsaI. antibodyDNA MTase antibodyDNA MTase HsaI antibodyDNMT 1 antibodyDNMT antibodyDnmt1 antibodyDnmt1 antibodyDNMT1_HUMAN antibodyDnmt1o antibodyFLJ16293 antibodyHSN1E antibodyM.HsaI antibodyMCMT antibodyMet1 antibodyMGC104992 antibodymMmul antibodyMommeD2 antibody

  see all

Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt1 antibody [60B1220] - ChIP Grade (ab13537)

  ab13537 at 1/10000 dilution staining Dnmt1 in mouse brain tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti-mouse secondary was used at 1/10 dilution.

• 

  Flow Cytometry-Dnmt1 antibody [60B1220.1] - ChIP Grade(ab13537)
  Overlay histogram showing HeLa cells stained with ab13537 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13537, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  
• 

  Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab13537)
  Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab13537) at 1/1000 dilution + 0.1ug mouse recombinant protein. Additional lower band is probably degraded or in other ways modified Dnmt1.
  
  Predicted band size : 183 kDa
  
• 

  Western blot - Dnmt1 antibody [60B1220] (ab13537)
  All lanes : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab13537) at 1 µg/ml
  
  Lane 1 : HCT116 whole cell lysate
  Lane 2 : HCT116 DNMT1 KO whole cell lysate
  Lane 3 : HCT116 DNMT3b KO whole cell lysate
  
  Secondary
  HRP conjugated Sheep anti-mouse IgG
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 183 kDa
  
  
  Exposure time : 1 minute

  This image is courtesy of an anonymous Abreview

  See Abreview

Protocols

• Western Blot Protocol