Recombinant Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139]
See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies -
Description
Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: Human breast carcinoma Tissue. Flow Cyt (Intra): HeLa, and C6 cells. ICC/IF: HeLa, and C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP139 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature.
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ICC/IF |
1/25.
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Flow Cyt (Intra) |
1/250.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval by boiling tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubate with primary antibody for 10 minutes at room temperature. |
ICC/IF
1/25. |
Flow Cyt (Intra)
1/250. |
Target
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Function
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. -
Involvement in disease
Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 424971 Chicken
- Entrez Gene: 282306 Cow
- Entrez Gene: 5290 Human
- Entrez Gene: 18706 Mouse
- Entrez Gene: 170911 Rat
- Omim: 171834 Human
- SwissProt: P32871 Cow
- SwissProt: P42336 Human
see all -
Alternative names
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
- 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
- caPI3K antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1/25 dilution (10 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing cytoplasmic staining in HeLa cells.
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Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab135384 has been referenced in 1 publication.
- Chen Z et al. Aspirin has a better effect on PIK3CA mutant colorectal cancer cells by PI3K/Akt/Raptor pathway. Mol Med 26:14 (2020). PubMed: 32000660