Anti-AIRE antibody - ChIP Grade (ab13573)
- Product nameAnti-AIRE antibody - ChIP GradeSee all AIRE primary antibodies ...
- DescriptionGoat polyclonal to AIRE - ChIP Grade
- SpecificityThis antibody is expected to recognize two of the tree reported isoforms (NP_000374 and NP_000649 but NOT NP_000650)
- Tested applicationsChIP, WB, IHC-P more details
- Species reactivityReacts with: Human
Synthetic peptide: QSMARPAAPFPS, corresponding to C terminal amino acids 534-545 of Human AIRE.
- General notesGenBank Accession Number - NP_000374, NP_000649
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab13573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||ChIP: Use at an assay dependent concentration.|
|WB||WB: Use a concentration of 0.01 - 0.03 µg/ml. Can be blocked with AIRE peptide (534-545) (ab23217).|
|IHC-P||IHC-P: Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
- FunctionTranscriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
- Tissue specificityWidely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
- Involvement in diseaseDefects in AIRE are a cause of autoimmune poly-endocrinopathy candidiasis ectodermal dystrophy (APECED) [MIM:240300]; also known as autoimmune polyglandular syndrome type I (APS-1). APECED is an autosomal recessive disease characterized by: (1) autoimmune polyendocrinopathies: hypoparathyroidism, adrenocortical failure, IDDM, gonadal failure, hypothyroidism, pernicious anemia, and hepatitis; (2) chronic mucocutaneous candidiasis; (3) ectodermal dystrophies: vitiligo, alopecia, keratopathy, dystrophy of dental enamel, nails and tympanic membranes. In addition, a high proportion of patients develop squamous cell carcinoma of the oral mucosa. The disease is reported worldwide but is exceptionally prevalent among the Finnish population (incidence 1:25000) and the Iranian jews (incidence 1:9000).
Note=Most of the mutations alter the nucleus-cytoplasm distribution of AIRE and disturb its association with nuclear dots and cytoplasmic filaments. Most of the mutations also decrease transactivation of the protein. The HSR domain is responsible for the homomultimerization activity of AIRE. All the missense mutations of the HSR and the SAND domains decrease this activity, but those in other domains do not. The AIRE protein is present in soluble high-molecular-weight complexes. Mutations in the HSR domain and deletion of PHD zinc fingers disturb the formation of these complexes.
- Sequence similaritiesContains 1 HSR domain.
Contains 2 PHD-type zinc fingers.
Contains 1 SAND domain.
- DomainThe L-X-X-L-L repeats may be implicated in binding to nuclear receptors.
The HSR domain is required for localization on tubular structures (N-terminal part) and for homodimerization.
Interacts via the first PHD domain with the N-terminus of histone H3 that is not methylated at 'Lys-4'. Disruption of the first PHD domain has been shown to lead to reduced transcriptional activity and to localization of the protein mainly in the cytoplasm in small granules. While the PHD zinc fingers are necessary for the transactivation capacity of the protein, other regions also modulate this function.
modificationsPhosphorylated. Phosphorylation could trigger oligomerization.
- Cellular localizationNucleus. Cytoplasm. Associated with tubular structures and in discrete nuclear dots resembling ND10 nuclear bodies. May shuttle between nucleus and cytoplasm.
- AIRE antibodyAIRE_HUMAN antibodyAIRE1 antibody
- APECED antibodyAPECED protein antibodyAPS1 antibodyAPSI antibodyAutoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein antibodyAutoimmune regulator antibodyAutoimmune regulator protein antibodyPGA1 antibody
Anti-AIRE antibody - ChIP Grade images
ab13573 (2µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
All lanes : Anti-AIRE antibody - ChIP Grade (ab13573) at 0.01 µg/ml
Lane 1 : HEK293 cell lysate, untransfected
Lane 2 : HEK293 cell lysate, transfected with human AIRE
ab13573 at 2.5 µg/ml staining Human Thymus by IHC-P. Thymic medula shows staining of select nuclei. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
References for Anti-AIRE antibody - ChIP Grade (ab13573)
This product has been referenced in:
- Žumer K et al. Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes. Mol Cell Biol 32:1354-62 (2012). WB . Read more (PubMed: 22310661) »
- Ruan QG et al. The autoimmune regulator directly controls the expression of genes critical for thymic epithelial function. J Immunol 178:7173-80 (2007). ChIP ; Mouse . Read more (PubMed: 17513766) »