HeLa Staurosporine Treated and Normal Cell Lysate Set (ab136806)

Overview

• Product nameHeLa Staurosporine Treated and Normal Cell Lysate Set
• Product overview

  Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases. It is commonly used to induce apoptosis in experimental settings, although the mechanism is not fully understood.
  The staurosporine-treated HeLa lysate is designed for use as a western blot positive control for antibodies against apoptosis-specific proteins or for experimental treatments to induce apoptosis

   

• Notes

  Confluent HeLa cells were treated with either the vehicle (DMSO, 0.2%) or staurosporine (1 uM) for 4 hours. Cells were pelleted then lysed with Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM NaF, 1x Protease Inhibitors, 1% SDS) and the lysate cleared by sonication. The protein concentration was measured by BCA assay. Modified 5x SDS sample buffer was added to the lysate before storage at -80ºC. The lysates were validated by western blotting with the ab136812 Apoptosis WB cocktail (cleaved PARP, actin, caspase 3).

• Tested applicationsWB, SDS-PAGE more details

Properties

• Storage instructionsStore at -80°C. Please refer to protocols.

• Components	2 units
  HeLa Staurosporine Treated Lysate	1 x 200µg
  HeLa Vehicle Treated Lysate	1 x 200µg

• Research Areas

Applications

HeLa Staurosporine Treated and Normal Cell Lysate Set images

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  Western Blot
  Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine, 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution