Anti-Histone H3 (phospho T6) antibody - ChIP Grade (ab14102)

Overview

• Product nameAnti-Histone H3 (phospho T6) antibody - ChIP GradeSee all Histone H3 primary antibodies ...
• Description
  Rabbit polyclonal to Histone H3 (phospho T6) - ChIP Grade
• Tested applicationsChIP, IHC-P, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, phosphorylated at T6.

  Read Abcam's proprietary immunogen policy

• Positive controlColcemid-treated HeLa histone Preparation; Hela whole cell extract

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Phosphorylated

Applications

Target

• FunctionVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Sequence similaritiesBelongs to the histone H3 family.
• Developmental stageExpressed throughout the cell cycle independently of DNA synthesis.
• Post-translational
  modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
  Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
  Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
  Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
  Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. Phosphorylation on Ser-32 (H3S31ph) is specific to regions bordering centromeres in metaphase chromosomes.
  Ubiquitinated. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination.
• Cellular localizationNucleus. Chromosome.

Target information above from: UniProt accession P84243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3020 Human
  • Entrez Gene: 3021 Human
  • Entrez Gene: 15078 Mouse
  • Entrez Gene: 15081 Mouse
  • Omim: 601128 Human
  • SwissProt: Q93081 Human
  • SwissProt: Q16695 Human
  • SwissProt: P84243 Human

  • SwissProt: P84244 Mouse
  • Unigene: 180877 Human
  • Unigene: 533624 Human
  • Unigene: 726012 Human
  • Unigene: 138832 Mouse
  • Unigene: 18516 Mouse
  • Unigene: 315189 Mouse
  • Unigene: 316825 Mouse
  • Unigene: 322735 Mouse
  • Unigene: 371563 Mouse
  • Unigene: 442502 Mouse

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• Alternative names
  H3 3 like sequence MH921 antibodyH3 3 like sequence MH921 antibodyH3 3A antibody

  H3 a antibodyH3 b antibodyH3 c antibodyH3 d antibodyH3 f antibodyH3 h antibodyH3 histone family member E pseudogene antibodyH3 histone family member E pseudogene antibodyH3 i antibodyH3 j antibodyH3 k antibodyH3 l antibodyH33_HUMAN antibodyH3F3 antibodyH3f3b antibodyHistone H3 3 pseudogene antibodyHistone H3 3 pseudogene antibodyHistone H3.3 antibody

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Anti-Histone H3 (phospho T6) antibody - ChIP Grade images

• 

  Western blot - Histone H3 (phospho T6) antibody (ab14102)
  All lanes : Anti-Histone H3 (phospho T6) antibody - ChIP Grade (ab14102) at 1 µg/ml
  
  Lane 1 : Colcemid treated histone
  Lane 2 : Untreated histone
  Lane 3 : Colcemid treated histone with Histone H3 peptide- phospho T6 (ab14103) at 1 µg/ml
  Lane 4 : Untreated histone with Histone H3 peptide- phospho T6 (ab14103) at 1 µg/ml
  Lane 5 : Colcemid treated histone with Histone H3 peptide - unmodified (ab2903) at 1 µg/ml
  Lane 6 : Untreated histone with Histone H3 peptide - unmodified (ab2903) at 1 µg/ml
  Lane 7 : Colcemid treated histone with Histone H3 peptide - phospho T3 (ab17578)
  Lane 8 : Untreated histone with Histone H3 peptide - phospho T3 (ab17578)
  Lane 9 : Colcemid treated histone with Histone H3 peptide - phospho T11 (ab24444)
  Lane 10 : Untreated histone with Histone H3 peptide - phospho T11 (ab24444)
  
  Lysates/proteins at 2.5 µg per lane.
  
  
  Predicted band size : 15 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Histone H3 (phospho T6) antibody (ab14102)This image is part of an abreview kindly submitted by Kirk McManus.
  ab14102 staining Histone H3 in HeLa cells, by ICC/IF, in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3^® (green). Cells counterstained with DAPI to show the nucleus (red).

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