Anti-Calreticulin antibody - ER Marker (ab14234)

Overview

• Product nameAnti-Calreticulin antibody - ER MarkerSee all Calreticulin primary antibodies ...
• Description
  Chicken polyclonal to Calreticulin - ER Marker
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Cow, Human
  Predicted to work with: a wide range of other species
• Immunogen

  Synthetic peptide: AEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKD EDEEDEEDKEEDEEEDVPGQAKDEL , corresponding to amino acids 353-416 of Human Calreticulin.

  Run BLAST with Run BLAST with
• Positive controlNRK cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgY
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • ER

Applications

Target

• FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
• Sequence similaritiesBelongs to the calreticulin family.
• DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
  The interaction with glycans occurs through a binding site in the globular lectin domain.
  The zinc binding sites are localized to the N-domain.
  Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
• Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.

Target information above from: UniProt accession P27797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281036 Cow
  • Entrez Gene: 811 Human
  • Entrez Gene: 12317 Mouse
  • Entrez Gene: 64202 Rat
  • Omim: 109091 Human
  • SwissProt: P52193 Cow
  • SwissProt: P27797 Human
  • SwissProt: Q3TVD2 Mouse

  • SwissProt: P14211 Mouse
  • SwissProt: P15253 Rabbit
  • SwissProt: P18418 Rat
  • Unigene: 515162 Human
  • Unigene: 1971 Mouse
  • Unigene: 467043 Mouse
  • Unigene: 974 Rat

  see all

• Alternative names
  Autoantigen RO antibodyCALR antibodyCALR protein antibody

  CALR_HUMAN antibodyCalregulin antibodyCalreticulin antibodycC1qR antibodyCRP55 antibodyCRT antibodyCRTC antibodyEndoplasmic reticulum resident protein 60 antibodyERp60 antibodyFLJ26680 antibodygrp60 antibodyHACBP antibodyRO antibodySicca syndrome antigen A antibodySSA antibody

  see all

Anti-Calreticulin antibody - ER Marker images

• 

  Western blot - Calreticulin antibody - ER Marker (ab14234)
  
  
  Predicted band size : 48 kDa
  
  
  Western blot analysis of Calreticulin in rat kidney NRK cells. ab14234 was used at a 1/2000 dilution using a colorimetric method for signal development.
• 

  Immunocytochemistry/ Immunofluorescence - Calreticulin antibody - ER Marker (ab14234)This image is courtesy of an Abreview submitted by Dr laurent barbe

  ab14234 at 1/800 staining human U251mg (glioma) cells by ICC/IF. The cells were formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat polyclonal antibody was used as the secondary.

  See Abreview