Anti-HA tag antibody [4C12] - ChIP Grade (ab1424)

Overview

• Product nameAnti-HA tag antibody [4C12] - ChIP GradeSee all HA tag primary antibodies ...
• Description
  Mouse monoclonal [4C12] to HA tag - ChIP Grade
• SpecificityThe antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
• Tested applicationsELISA, ChIP, ICC, IP, WB more details

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
• Concentration information loading...
• Clonality Monoclonal
• Clone number4C12
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Epitope Tags
  • HA Tag

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

Applications

Target

• RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

• Alternative names
  HA epitope tag antibodyhemagglutinin antibody

Anti-HA tag antibody [4C12] - ChIP Grade images

• 

  Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)This image was kindly supplied as part of the review submitted by one of our customers.
  This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunoflourescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
• 

  ChIP - HA tag antibody [4C12] (ab1424)

  Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

• 

  Western blot - Anti-HA tag antibody [4C12] - ChIP Grade (ab1424)
  Anti-HA tag antibody [4C12] - ChIP Grade (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
  
  Secondary
  HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 5 seconds

  This image is courtesy of an anonymous Abreview

  See Abreview