Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)
Overview
- Product nameAnti-Bmi1 antibody [1.T.21] - ChIP GradeSee all Bmi1 primary antibodies ...
- DescriptionMouse monoclonal [1.T.21] to Bmi1 - ChIP Grade
- SpecificityRecognizes mouse Bmi-1(triplet).
- Tested applicationsICC/IF, Flow Cyt, ChIP, WB, ICC, IP, IHC-Fr, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Cat, Human, Zebrafish
- Immunogen
Recombinant fragment, corresponding to amino acids 1-202 of Mouse Bmi1
- Positive controlU2OS whole cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.15M Tris-glycine. pH 7.4 -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- Clone number1.T.21
- IsotypeIgG1
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab14389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 0.65 µg/ml. PubMed: 19001505 |
| Flow Cyt | Flow Cyt: Use a concentration of 0.65 µg/ml. PubMed: 19001505 |
| ChIP | ChIP: Use at an assay dependent dilution. PubMed: 18332116 |
| WB | WB: Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab14389 0.2ug/ml. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system. |
| ICC | ICC: Use at an assay dependent dilution. |
| IP | IP: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. PubMed: 17210912 |
| IHC-P | IHC-P: 1/100. |
Target
- FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
- Sequence similaritiesContains 1 RING-type zinc finger.
- Post-translational
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. - Cellular localizationNucleus. Cytoplasm.
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Database links
- Entrez Gene: 554342 Cat
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 307151 Rat
- Entrez Gene: 321505 Zebrafish
- Omim: 164831 Human
- SwissProt: Q9TST0 Cat
- SwissProt: P35226 Human
- SwissProt: P25916 Mouse
- Unigene: 380403 Human
- Unigene: 289584 Mouse
see all
Target information above from: UniProt accession
P35226
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody
- BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibody
see all
Anti-Bmi1 antibody [1.T.21] - ChIP Grade images
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![Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)](http://a.abcam.com/ps/datasheet/Images/14/ab14389/ab14389_1.jpg)
Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)Darin McDonald, Cross Cancer InstituteSK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.55 Triton-X100 and incubated for 1 hour with ab14389 (1/200). The Bmi1 staining is shown in red. The cells were counterstained with DAPI (blue). 100x magnification.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)](http://a.abcam.com/ps/datasheet/images/14/ab14389/Bmi1-Primary-antibodies-ab14389-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)Image from Ganguli-Indra G et al, PLoS One. 2009;4(4):e5367. Epub 2009 Apr 28, Fig 2.ab14389 staining Bmi1 in human head and neck squamous cell cancers (HNSCC) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Sections were deparaffinized in xylene, dehydrated through graded alcohols, and placed in 0.1% hydrogen peroxide to quench any endogenous peroxidase activity. A 5 minute, 750 W microwave pretreatment in citrate buffer (pH 6.0) was repeated 4 times and followed by treatment with 10% normal rabbit serum for 30 minutes to block nonspecific antibody binding. The slides were then incubated with ab14389 at a 1/100. Primary antibody incubation was followed by three washes with PBST and incubation with fluorescently-labeled Cy2 (1/250) secondary antibody for 2 hours. Nuclei were counterstained with DAPI. Finally, sections were rinsed with PBST, dehydrated through sequential washes in 50%, 70%, 95%, and 100% ethanol and then cleared in xylene. Slides were mounted with DPX mounting media and allowed to dry overnight. -
![Western blot - Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)](http://a.abcam.com/ps/datasheet/images/14/ab14389/Bmi1-Primary-antibodies-ab14389-28.jpg)
Western blot - Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389) at 1/500 dilution + whole cell lysate prepared from Panc1 cancer cells at 35 µg
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 27 kDa,48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutesImage courtesy of an anonymous Abreview.
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![Western blot - Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)](http://a.abcam.com/ps/datasheet/images/14/ab14389/Bmi1-Primary-antibodies-ab14389-30.jpg)
Western blot - Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389) at 1/500 dilution + whole tissue lysate prepared from murine pancreatic cancer at 50 µg
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 15 minutesImage courtesy of an anonymous Abreview.
References for Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)
This product has been referenced in:
- Balasubramanian S et al. A Proteasome Inhibitor-stimulated Nrf1 Protein-dependent Compensatory Increase in Proteasome Subunit Gene Expression Reduces Polycomb Group Protein Level. J Biol Chem 287:36179-89 (2012). WB . Read more (PubMed: 22932898) »
- Kim HG et al. Gene silencing in HIV-1 latency by polycomb repressive group. Virol J 8:179 (2011). IP ; Human . Read more (PubMed: 21496352) »
