Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
- Product nameAnti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial MarkerSee all SDHA primary antibodies ...
- DescriptionMouse monoclonal [2E3GC12FB2AE2] to SDHA - Mitochondrial Marker
- Tested applicationsICC, IHC-Fr, Flow Cyt, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human, Caenorhabditis elegans
Purified mitochondrial complex II (Cow).
- Positive controlHuman heart mitochondria.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: HEPES buffered saline
- Concentration information loading...
- PurityIgG fraction
- Purification notesNear homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Clonality Monoclonal
- Clone number2E3GC12FB2AE2
- Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab14715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||ICC: Use a concentration of 0.2 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use a concentration of 1 µg/ml.|
|WB||WB: Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||IHC-P: Use at an assay dependent dilution. PubMed: 20484225|
- FunctionFlavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
- PathwayCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
- Involvement in diseaseDefects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
- Sequence similaritiesBelongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
- Cellular localizationMitochondrion inner membrane.
- Entrez Gene: 281480 Cow
- Entrez Gene: 478634 Dog
- Entrez Gene: 6389 Human
- Entrez Gene: 66945 Mouse
- Entrez Gene: 157074 Rat
- Omim: 600857 Human
- SwissProt: Q09508 Caenorhabditis elegans
- SwissProt: P31039 Cow
- SwissProt: P31040 Human
- SwissProt: Q8K2B3 Mouse
- SwissProt: Q920L2 Rat
- Unigene: 440475 Human
- Unigene: 158231 Mouse
- Unigene: 101725 Rat
- CMD1GG antibodyDHSA_HUMAN antibodyFlavoprotein subunit of complex II antibody
- Fp antibodyPGL5 antibodySDH 2 antibodySDH1 antibodySDH2 antibodySDHA antibodySDHF antibodySuccinate dehydrogenase [ubiquinone] flavoprotein subunit antibodySuccinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibodySuccinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibodySuccinate dehydrogenase complex flavoprotein subunit antibodySuccinate dehydrogenase complex flavoprotein subunit precursor antibodySuccinate dehydrogenase complex subunit A antibodySuccinate Dehydrogenase Complex subunit A Flavoprotein antibody
Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker images
ICC/IF image of ab14715 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14715, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
ab14715 (2µg/ml) staining SDHA in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining within the seminal vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : Isolated mitochondria from HepG2 at 20 µg
Predicted band size : 70 kDa
Observed band size : 70 kDa
Mitochondrial localization of complex II visualized by immunocytochemistry using ab14715. Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14715 (0.2 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (2 µg/ml).
Skeletal muscle immunohistochemistry using ab14715. Fixed frozen tissue sections from a patient with a single large deletion of the mtDNA were used. All muscle fibers exhibit complex II immunoreactivity, consistent with the nuclear DNA-encoded expression pattern of this and all other subunits of complex II.
HL-60 cells were stained with 1 µg/mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
This product has been referenced in:
- Kumarasamy S et al. Construction of two novel reciprocal conplastic rat strains and characterization of cardiac mitochondria. Am J Physiol Heart Circ Physiol 304:H22-32 (2013). WB ; Rat . Read more (PubMed: 23125210) »
- Houstek J et al. Nonsynonymous variants in mt-Nd2, mt-Nd4, and mt-Nd5 are linked to effects on oxidative phosphorylation and insulin sensitivity in rat conplastic strains. Physiol Genomics 44:487-94 (2012). WB ; Rat . Read more (PubMed: 22414913) »