PC-12 nuclear extract lysate (hypoxia treated) (ab14886)
Overview
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Product name
PC-12 nuclear extract lysate (hypoxia treated)
See all PC12 lysates -
General notes
This PC-12 extract (Hypoxia) was tested positively in Western blot and EMSA for NFKB p50 and Pax-3. Before harvesting the cells were deprived of oxygen for 4 hours. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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Research areas
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Background
Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab14886 has not yet been referenced specifically in any publications.