Anti-14-3-3 beta antibody (ab15260)

Overview

• Product nameAnti-14-3-3 beta antibodySee all 14-3-3 beta primary antibodies ...
• Description
  Rabbit polyclonal to 14-3-3 beta
• Tested applicationsICC/IF, WB, IP, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

• Positive controlTested with NIH/3T3 cells, prostate cancer cells and liver tumor cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • 14-3-3

  • Signal Transduction
  • Protein Phosphorylation
  • pSer / pThr

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
  Isoform Short contains a N-acetylmethionine at position 1.
• Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P31946 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7529 Human
  • Entrez Gene: 54401 Mouse
  • Entrez Gene: 56011 Rat
  • Omim: 601289 Human
  • SwissProt: P31946 Human
  • SwissProt: Q9CQV8 Mouse
  • SwissProt: P35213 Rat
  • Unigene: 643544 Human

  • Unigene: 34319 Mouse
  • Unigene: 485025 Mouse
  • Unigene: 8653 Rat

  see all

• Alternative names
  14 3 3 protein beta/alpha antibody14-3-3 protein beta/alpha antibody1433B_HUMAN antibody

  Brain protein 14 3 3 beta isoform antibodyGW 128 antibodyGW128 antibodyHS 1 antibodyHS1 antibodyKCIP 1 antibodyKCIP-1 antibodyKCIP1 antibodyN-terminally processed antibodyProtein 1054 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide antibodyYWHAA antibodyYWHAB antibody

  see all

Anti-14-3-3 beta antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 beta antibody (ab15260)

  ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).

• 

  Western blot - 14-3-3 beta antibody (ab15260)
  Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate
  
  Predicted band size : 28 kDa
  Observed band size : 28 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)
  ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.