Anti-beta Tubulin antibody (ab15568)

Overview

• Product nameAnti-beta Tubulin antibodySee all beta Tubulin primary antibodies ...
• Description
  Rabbit polyclonal to beta Tubulin
• Tested applicationsFlow Cyt, ICC/IF, IHC-P, IP, WB more details
• Species reactivity
  Reacts with: Mouse, Cow, Human
  Predicted to work with: Rat, Chicken, Guinea pig, Pig, Saccharomyces cerevisiae, Gerbil, Sea Urchin, Fungi
• Immunogen

  Synthetic peptide: NDLVSEYQQYQDATA, corresponding to amino acids 416-430 of Human beta Tubulin.

  Run BLAST with Run BLAST with
• Positive controlMAD109 cells, Skin and lung, HeLa cell line

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: Sodium Azide
  Constituents: 10mM PBS, BSA. pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • Tubulin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Microtubules

Applications

Target

• FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
• Tissue specificityUbiquitously expressed with highest levels in spleen, thymus and immature brain.
• Sequence similaritiesBelongs to the tubulin family.
• DomainThe highly acidic C-terminal region may bind cations such as calcium.
• Post-translational
  modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
• Cellular localizationCytoplasm > cytoskeleton.

Target information above from: UniProt accession P07437 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396254 Chicken
  • Entrez Gene: 533307 Cow
  • Entrez Gene: 203068 Human
  • Entrez Gene: 22154 Mouse
  • Entrez Gene: 733686 Pig
  • Entrez Gene: 29214 Rat
  • Omim: 191130 Human
  • SwissProt: Q2KJD0 Cow

  • SwissProt: P07437 Human
  • SwissProt: P99024 Mouse
  • SwissProt: Q767L7 Pig
  • SwissProt: P69897 Rat
  • Unigene: 636480 Human
  • Unigene: 714425 Human
  • Unigene: 273538 Mouse
  • Unigene: 2458 Rat

  see all

• Alternative names
  Beta 4 tubulin antibodyBeta 5 tubulin antibodybeta Ib tubulin antibody

  Beta1 tubulin antibodyClass I beta tubulin antibodyM40 antibodyMGC117247 antibodyMGC16435 antibodyOK/SW cl.56 antibodyOK/SWcl.56 antibodyTBB5_HUMAN antibodyTUBB 1 antibodyTUBB 2 antibodyTUBB 5 antibodyTUBB antibodyTUBB1 antibodyTUBB2 antibodyTUBB5 antibodytubulin beta 1 chain antibodyTubulin beta 2 chain antibodytubulin beta 5 chain antibodyTubulin beta chain antibodyTubulin beta class I antibodyTubulin beta polypeptide antibodyTubulin beta-2 chain antibodyTubulin beta-5 chain antibody

  see all

Anti-beta Tubulin antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody (ab15568)

  ab15568 staining beta Tubulin in human lung by Immunohistochemistry (FFPE-sections).

• 

  Western blot - beta Tubulin antibody (ab15568)
  All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/ml
  
  Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size : 55 kDa
  Observed band size : 55 kDa
  
• 

  Western blot
  Anti-beta Tubulin antibody (ab15568) at 1 µg/ml + beta Tubulin protein (Tagged) (ab70187) at 0.1 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 30 seconds
• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody (ab15568)
  ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.