Anti-Iba1 antibody [1022-5] (FITC) - Microglial marker (ab15691)

Overview

• Product nameAnti-Iba1 antibody [1022-5] (FITC) - Microglial markerSee all Iba1 primary antibodies ...
• Description
  Mouse monoclonal [1022-5] to Iba1 - Microglial marker (FITC)
• ConjugationFITC
• Tested applicationsFlow Cyt more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Recombinant full length protein (Human)

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number1022-5
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Immunology
  • Immune Interventions
  • Transplantation

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Microglia marker

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
• Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
• Sequence similaritiesContains 2 EF-hand domains.
• Post-translational
  modificationsPhosphorylated on serine residues.
• Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Target information above from: UniProt accession P55008 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 199 Human
  • Entrez Gene: 29427 Rat
  • Omim: 601833 Human
  • SwissProt: P55008 Human
  • SwissProt: P55009 Rat
  • Unigene: 76364 Human
  • Unigene: 32080 Rat

• Alternative names
  AIF 1 antibodyAIF 1 antibodyAIF-1 antibody

  AIF1 antibodyAIF1 protein antibodyAIF1_HUMAN antibodyallograft inflammatory factor 1 antibodyAllograft inflammatory factor 1 splice variant G antibodyallograft inflammatory factor-1 splice variant Hara-1 antibodyballoon angioplasty responsive transcription antibodyBART 1 antibodyG1 antibodyG1 putative splice variant of allograft inflamatory factor 1 antibodyIBA 1 antibodyIBA 1 antibodyIBA1 antibodyinterferon gamma responsive transcript antibodyinterferon responsive transcript factor 1 antibodyIonized calcium binding adapter molecule 1 antibodyIonized calcium-binding adapter molecule 1 antibodyionized calcium-binding adapter molecule antibodyIRT 1 antibodyIRT 1 antibodyIRT1 antibodyMicroglia response factor antibodyMRF1 antibodyProtein G1 antibody

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Anti-Iba1 antibody [1022-5] (FITC) - Microglial marker images

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  Flow Cytometry-Iba1 antibody [1022-5] (FITC) - Microglial marker(ab15691)
  Overlay histogram showing THP1 cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10µl/1x10⁶ cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10µl/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.